| RRC ID |
58840
|
| 著者 |
Uchikawa M, Kato M, Nagata A, Sanada S, Yoshikawa Y, Tsunematsu Y, Sato M, Suzuki T, Hashidume T, Watanabe K, Yoshikawa Y, Miyoshi N.
|
| タイトル |
Elevated levels of proinflammatory volatile metabolites in feces of high fat diet fed KK-Ay mice.
|
| ジャーナル |
Sci Rep
|
| Abstract |
When the microfloral composition deteriorates, it triggers low-level chronic inflammation associated with several lifestyle-related diseases including obesity and diabetic mellitus. Fecal volatile organic compounds (VOCs) have been found to differ in gastrointestinal diseases as well as intestinal infection. In this study, to evaluate a potential association between the pathogenesis of lifestyle-related diseases and VOCs in the intestinal tract, fecal VOCs from obese/diabetic KK-Ay mice (KK) or controls (C57BL/6J mice; BL) fed a normal or high fat diet (NFD or HFD) were investigated using headspace sampler-GC-EI-MS. Principal component analysis (PCA) of fecal VOC profiles clearly separated the experimental groups depending on the mouse lineage (KK vs BL) and the diet type (NFD vs HFD). 16 s rRNA sequencing revealed that the PCA distribution of VOCs was in parallel with the microfloral composition. We identified that some volatile metabolites including n-alkanals (nonanal and octanal), acetone and phenol were significantly increased in the HFD and/or KK groups. Additionally, these volatile metabolites induced proinflammatory activity in the RAW264 murine macrophage cell line indicating these bioactive metabolites might trigger low-level chronic inflammation. These results suggest that proinflammatory VOCs detected in HFD-fed and/or diabetic model mice might be novel noninvasive diagnosis biomarkers for diabetes.
|
| 巻・号 |
10(1)
|
| ページ |
5681
|
| 公開日 |
2020-3-30
|
| DOI |
10.1038/s41598-020-62541-7
|
| PII |
10.1038/s41598-020-62541-7
|
| PMID |
32231228
|
| PMC |
PMC7105489
|
| MeSH |
Animals
Biomarkers / analysis
Diet, High-Fat / adverse effects*
Disease Models, Animal
Feces / chemistry
Gastrointestinal Microbiome / genetics
Inflammation
Male
Mice
Mice, Inbred C57BL
Mice, Obese
Obesity / metabolism*
RAW 264.7 Cells
RNA, Ribosomal, 16S / genetics
Volatile Organic Compounds / analysis*
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
