RRC ID |
59272
|
著者 |
Karpf S, Riche CT, Di Carlo D, Goel A, Zeiger WA, Suresh A, Portera-Cailliau C, Jalali B.
|
タイトル |
Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates.
|
ジャーナル |
Nat Commun
|
Abstract |
Two-Photon Microscopy has become an invaluable tool for biological and medical research, providing high sensitivity, molecular specificity, inherent three-dimensional sub-cellular resolution and deep tissue penetration. In terms of imaging speeds, however, mechanical scanners still limit the acquisition rates to typically 10-100 frames per second. Here we present a high-speed non-linear microscope achieving kilohertz frame rates by employing pulse-modulated, rapidly wavelength-swept lasers and inertia-free beam steering through angular dispersion. In combination with a high bandwidth, single-photon sensitive detector, this enables recording of fluorescent lifetimes at speeds of 88 million pixels per second. We show high resolution, multi-modal - two-photon fluorescence and fluorescence lifetime (FLIM) - microscopy and imaging flow cytometry with a digitally reconfigurable laser, imaging system and data acquisition system. These high speeds should enable high-speed and high-throughput image-assisted cell sorting.
|
巻・号 |
11(1)
|
ページ |
2062
|
公開日 |
2020-4-28
|
DOI |
10.1038/s41467-020-15618-w
|
PII |
10.1038/s41467-020-15618-w
|
PMID |
32346060
|
PMC |
PMC7188897
|
MeSH |
Euglena / cytology
Imaging, Three-Dimensional
Microscopy, Fluorescence, Multiphoton*
Time Factors
|
IF |
11.878
|
引用数 |
1
|
リソース情報 |
藻類 |
NIES-48 |