| RRC ID |
59348
|
| 著者 |
Gómez-Orte E, Sáenz-Narciso B, Zheleva A, Ezcurra B, de Toro M, López R, Gastaca I, Nilsen H, Sacristán MP, Schnabel R, Cabello J.
|
| タイトル |
Disruption of the Caenorhabditis elegans Integrator complex triggers a non-conventional transcriptional mechanism beyond snRNA genes.
|
| ジャーナル |
PLoS Genet
|
| Abstract |
Gene expression is generally regulated by recruitment of transcription factors and RNA polymerase II (RNAP II) to specific sequences in the gene promoter region. The Integrator complex mediates processing of small nuclear RNAs (snRNAs) as well as the initiation and release of paused RNAP II at specific genes in response to growth factors. Here we show that in C. elegans, disruption of the Integrator complex leads to transcription of genes located downstream of the snRNA loci via a non-conventional transcription mechanism based on the lack of processing of the snRNAs. RNAP II read-through generates long chimeric RNAs containing snRNA, the intergenic region and the mature mRNA of the downstream gene located in sense. These chimeric sn-mRNAs remain as untranslated long non-coding RNAs, in the case of U1- and U2-derived sn-mRNAs, but can be translated to proteins in the case of SL-derived sn-mRNAs. The transcriptional effect caused by disruption of the Integrator complex is not restricted to genes located downstream of the snRNA loci but also affects key regulators of signal transduction such as kinases and phosphatases. Our findings highlight that these transcriptional alterations may be behind the correlation between mutations in the Integrator complex and tumor transformation.
|
| 巻・号 |
15(2)
|
| ページ |
e1007981
|
| 公開日 |
2019-2-1
|
| DOI |
10.1371/journal.pgen.1007981
|
| PII |
PGENETICS-D-18-01081
|
| PMID |
30807579
|
| PMC |
PMC6390993
|
| MeSH |
Animals
Animals, Genetically Modified
Caenorhabditis elegans / embryology
Caenorhabditis elegans / genetics*
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism
Down-Regulation
Gene Knockdown Techniques
Genes, Helminth
Mutation
RNA Polymerase II / genetics
RNA Polymerase II / metabolism
RNA Processing, Post-Transcriptional
RNA, Helminth / genetics*
RNA, Helminth / metabolism*
RNA, Small Nuclear / genetics*
RNA, Small Nuclear / metabolism*
Signal Transduction
Transcription Factors / genetics
Transcription Factors / metabolism
Transcription, Genetic
Up-Regulation
|
| IF |
5.224
|
| 引用数 |
4
|
| リソース情報 |
| 線虫 |
tm1615 |
