RRC ID |
60573
|
著者 |
Sato S, Fukuoka T, Saika A, Koshiyama T, Morita T.
|
タイトル |
A New Screening Approach for Glycolipid-type Biosurfactant Producers Using MALDI-TOF/MS.
|
ジャーナル |
J Oleo Sci
|
Abstract |
We applied matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) to screen for glycolipid-type biosurfactant (BS) producers. A crude extract of Pseudozyma antarctica, a well-known mannosylerythritol lipid (MEL) producer, was initially subjected to MALDI-TOF/MS. The spectrum of the extract showed the accumulation of diacylated MELs in culture. We then screened 80 environmental samples for BS-producing yeasts, and extracts from broth cultures of the selected five strains were examined using MALDI-TOF/MS. The results showed that all five strains produced MELs, whereas four strains also produced cellobiose lipids (CLs). By D1/D2 region sequence analysis, the MEL-producing strain was assigned to P. antarctica while the four MEL- and CL-producing strains were assigned to P. hubeiensis. These results demonstrate that MALDI-TOF/MS is a rapid and reliable tool to detect BS molecules in crude extracts of broth cultures to screen for glycolipid-type BS producers.
|
巻・号 |
68(12)
|
ページ |
1287-1294
|
公開日 |
2019-1-1
|
DOI |
10.5650/jos.ess19177
|
PMID |
31787677
|
MeSH |
Glycolipids / analysis*
Phylogeny
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
Surface-Active Agents / analysis*
Ustilaginales / chemistry
Ustilaginales / classification
Ustilaginales / genetics
|
IF |
1.304
|
引用数 |
0
|
リソース情報 |
一般微生物 |
JCM10317 |