| RRC ID |
61188
|
| 著者 |
Kume A, Ito K, Ueda Y, Hasegawa M, Urabe M, Mano H, Ozawa K.
|
| タイトル |
A G-CSF receptor-gyrase B fusion gene: A new type of molecular switch for expansion of genetically modified hematopoietic cells.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
We have developed a novel system for expansion of transduced hematopoietic cells. This system involves "selective amplifier genes" encoding fusion proteins between the granulocyte colony-stimulating factor receptor (Gcr) and the estrogen receptor (Er). The GcrEr chimeric gene conferred estrogen-dependent growth ability on murine hematopoietic cells. Here, we constructed a modified "selective amplifier gene" to circumvent possible concerns with the Er/estrogen switching system. The bacterial gyrase B (Gyr) gene was fused to the Gcr gene, and the GcrGyr fusion construct was introduced into interleukin-3 (IL-3)-dependent Ba/F3 cells. The dimeric antibiotic coumermycin induced IL-3-independent growth in Ba/F3 cells expressing GcrGyr. This stimulatory effect was antagonized by an excess amount of novobiocin, a monomeric form of coumermycin. These results suggest the feasibility of using Gyr as a molecular switch to regulate a growth signal in hematopoietic cells.
|
| 巻・号 |
260(1)
|
| ページ |
9-12
|
| 公開日 |
1999-6-24
|
| DOI |
10.1006/bbrc.1999.0859
|
| PII |
S0006-291X(99)90859-8
|
| PMID |
10381335
|
| MeSH |
Aminocoumarins
Animals
Anti-Bacterial Agents / pharmacology
Artificial Gene Fusion
Cell Count
Cell Culture Techniques
Cell Line
Chimera
Coumarins / pharmacology
DNA Gyrase
DNA Topoisomerases, Type II / genetics*
Gene Expression Regulation*
Hematopoietic Stem Cells / metabolism*
Mice
Novobiocin / pharmacology
Receptors, Granulocyte Colony-Stimulating Factor / genetics*
Time Factors
Transfection
|
| IF |
2.985
|
| リソース情報 |
| ヒト・動物細胞 |
Ba/F3(RCB0805) |
