RRC ID |
61473
|
著者 |
Mukai R, Kawabata K, Otsuka S, Ishisaka A, Kawai Y, Ji ZS, Tsuboi H, Terao J.
|
タイトル |
Effect of quercetin and its glucuronide metabolite upon 6-hydroxydopamine-induced oxidative damage in Neuro-2a cells.
|
ジャーナル |
Free Radic Res
|
Abstract |
Quercetin is ubiquitously distributed in plant foods. This antioxidative polyphenol is mostly converted to conjugated metabolites in the body. Parkinson disease (PD) has been suggested to be related to oxidative stress derived from abnormal dopaminergic activity. We evaluated if dietary quercetin contributes to the antioxidant network in the central nervous system from the viewpoint of PD prevention. A neurotoxin, 6-hydroxydopamine (6-OHDA), was used as a model of PD. 6-OHDA-induced H₂O₂ production and cell death in mouse neuroblastoma, Neuro-2a. Quercetin aglycone suppressed 6-OHDA-induced H₂O₂ production and cell death, although aglycone itself reduced cell viability at higher concentration. Quercetin 3-O-β-D-glucuronide (Q3GA), which is an antioxidative metabolite of dietary quercetin, was little incorporated into the cell resulting in neither suppression of 6-OHDA-induced cell death nor reduction of cell viability. Q3GA was found to be deconjugated to quercetin by microglial MG-6 cells. These results indicate that quercetin metabolites should be converted to their aglycone to exert preventive effect on damage to neuronal cells.
|
巻・号 |
46(8)
|
ページ |
1019-28
|
公開日 |
2012-8-1
|
DOI |
10.3109/10715762.2012.673720
|
PMID |
22404304
|
MeSH |
Animals
Antioxidants / pharmacology*
Biological Transport
Cell Death
Cell Line, Tumor
Cell Survival
Dose-Response Relationship, Drug
Hydrogen Peroxide / metabolism
Mice
Neurons / cytology
Neurons / drug effects*
Neurons / metabolism
Oxidation-Reduction
Oxidative Stress
Oxidopamine / pharmacology*
Quercetin / analogs & derivatives*
Quercetin / metabolism
Quercetin / pharmacology
|
IF |
2.839
|
リソース情報 |
ヒト・動物細胞 |
MG6(RCB2403) |