RRC ID 6159
Author Kusube M, Nishino T, Nishikawa Y, Goto M, Matsuki H, Iwahashi H.
Title Recovery of tobacco BY-2 cells after high hydrostatic pressure treatment.
Journal Ann N Y Acad Sci
Abstract The recovery of Nicotiana tabacum L. cv. Bright Yellow 2 (BY-2) cells in Linsmaire and Skoog medium after treatment at high hydrostatic pressure was investigated using an Evans Blue staining method to discriminate live from dead cells. The survival of BY-2 cells just after the high-pressure treatment at 5 degrees C and 25 degrees C decreased abruptly at pressures higher than 50 MPa and 100 MPa, respectively. Furthermore, almost all of the BY-2 cells treated at 5 degrees C and 25 degrees C recovered pressures below 25 MPa and 75 MPa, respectively. However, no BY-2 cells recovered at pressures above 100 MPa at either temperature.
Volume 1189
Pages 139-42
Published 2010-2-1
DOI 10.1111/j.1749-6632.2009.05185.x
PII NYAS5185
PMID 20233380
MeSH Biotechnology Cell Line Cell Proliferation Cell Survival Hydrostatic Pressure* Preservation, Biological / methods* Temperature Tobacco / cytology*
IF 4.728
Times Cited 0
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Resource
Arabidopsis / Cultured plant cells, genes rpc00001