| RRC ID |
61658
|
| 著者 |
Fujimori S, Hinoi E, Yoneda Y.
|
| タイトル |
Functional GABA(B) receptors expressed in cultured calvarial osteoblasts.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
In immature and mature primary cultured rat calvarial osteoblasts, both mRNA and corresponding proteins were constitutively expressed for 2 splice variants of GABA(B) receptor (GABA(B)R) subunits but not for any known GABA(A) and GABA(C) receptor subunits. The agonist for GABA(B)R baclofen significantly inhibited cAMP formation induced by forskolin in a manner sensitive to the antagonist 2-hydroxysaclofen. Similar expression was seen with mRNA for GABA(B)R-1a and -1b splice variants in the murine calvarial osteoblast cell line MC3TC-E1 cells cultured for 7-21 days in vitro (DIV). In these MC3T3-E1 cells, baclofen not only inhibited the activity of alkaline phosphatase, but also exacerbated Ca2+ accumulation, throughout the culture period up to 28 DIV. These results suggest that GABA may play an unidentified role in mechanisms associated with cellular proliferation, differentiation, and/or development through functional GABA(B)R constitutively expressed in cultured osteoblasts.
|
| 巻・号 |
293(5)
|
| ページ |
1445-52
|
| 公開日 |
2002-5-24
|
| DOI |
10.1016/S0006-291X(02)00405-9
|
| PII |
S0006-291X(02)00405-9
|
| PMID |
12054677
|
| MeSH |
3T3 Cells
Alkaline Phosphatase / metabolism
Alternative Splicing
Animals
Baclofen / pharmacology
Calcium / metabolism
Cells, Cultured
Cyclic AMP / metabolism
DNA, Complementary / metabolism
GABA Agonists / pharmacology
Immunoblotting
Mice
Osteoblasts / metabolism*
Protein Structure, Tertiary
Rats
Rats, Wistar
Receptors, GABA-B / biosynthesis*
Receptors, GABA-B / chemistry*
Receptors, GABA-B / genetics
Reverse Transcriptase Polymerase Chain Reaction
Skull / cytology*
Time Factors
gamma-Aminobutyric Acid / metabolism
|
| IF |
2.985
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
