論文 - 詳細
| RRC ID | 62109 |
|---|---|
| 著者 | Zhu DY, Guo QS, Li YL, Cui B, Guo J, Liu JX, Li P. |
| タイトル | Twist1 correlates with poor differentiation and progression in gastric adenocarcinoma via elevation of FGFR2 expression. |
| ジャーナル | World J Gastroenterol |
| Abstract |
AIM:To explore the correlation between Twist-related protein (Twist)1, fibroblast growth factor receptor (FGFR)2 and gastric adenocarcinoma differentiation and progression. METHODS:We evaluated Twist1 and FGFR2 in 52 gastric adenocarcinoma samples by immunohistochemistry and quantitative real time polymerase chain reaction, and analyzed the correlation between Twist1, FGFR2 and cancer differentiation. We also detected Twist1 and FGFR2 expression in gastric adenocarcinoma cell lines, and evaluated Twist1 influence on FGFR2 expression. In addition, we studied the role of FGFR2 in Twist1-promoted cancer progression, including proliferation, invasion and epithelial-mesenchymal transition (EMT). RESULTS:Twist1 and FGFR2 were detected in almost all the gastric adenocarcinoma samples. Twist1 (P = 0.0213) and FGFR2 (P = 0.0310) mRNA levels had a significant association with gastric adenocarcinoma differentiation. Moreover, Twist1 and FGFR2 expression in poorly differentiated cells (SNU-1 and SNU-16) was notably higher than in well-differentiated cells (MKN-7 and MKN-28). In poorly differentiated gastric adenocarcinomas, FGFR2 mRNA level was significantly positively correlated with Twist1 mRNA level (P = 0.004). Twist1 was proved to promote FGFR2 by regulating Twist1 expression by knockdown and overexpression. Additionally, Twist1 could induce proliferation, invasion and EMT in gastric cancer; of these, FGFR2 was required for invasion and EMT, rather than proliferation. CONCLUSION:Twist1 and FGFR2 are highly associated with differentiation of gastric adenocarcinoma; Twist1 can facilitate invasion and EMT in gastric adenocarcinoma via promotion of FGFR2 expression. |
| 巻・号 | 20(48) |
| ページ | 18306-15 |
| 公開日 | 2014-12-28 |
| DOI | 10.3748/wjg.v20.i48.18306 |
| PMID | 25561797 |
| PMC | PMC4277967 |
| MeSH | Adenocarcinoma / genetics Adenocarcinoma / metabolism* Adenocarcinoma / pathology Biomarkers, Tumor / genetics Biomarkers, Tumor / metabolism* Cell Differentiation* Cell Line, Tumor Cell Movement Cell Proliferation Disease Progression Epithelial-Mesenchymal Transition Gene Expression Regulation, Neoplastic Humans Immunohistochemistry Neoplasm Invasiveness Nuclear Proteins / genetics Nuclear Proteins / metabolism* Prognosis RNA Interference RNA, Messenger / metabolism Real-Time Polymerase Chain Reaction Receptor, Fibroblast Growth Factor, Type 2 / genetics Receptor, Fibroblast Growth Factor, Type 2 / metabolism* Stomach Neoplasms / genetics Stomach Neoplasms / metabolism* Stomach Neoplasms / pathology Transfection Twist-Related Protein 1 / genetics Twist-Related Protein 1 / metabolism* Up-Regulation |
| IF | 3.665 |
| リソース情報 | |
| ヒト・動物細胞 | MKN7(RCB0999) MKN28(RCB1000) |