| RRC ID |
62147
|
| 著者 |
Yoshimoto K, Tanaka C, Moritani M, Shimizu E, Yamaoka T, Yamada S, Sano T, Itakura M.
|
| タイトル |
Infrequent detectable somatic mutations of the RET and glial cell line-derived neurotrophic factor (GDNF) genes in human pituitary adenomas.
|
| ジャーナル |
Endocr J
|
| Abstract |
RET is a receptor tyrosine kinase expressed in neuroendocrine cells and tumors. RET is activated by a ligand complex comprising glial cell line-derived neurotrophic factor (GDNF) and GDNF receptor-alpha (GDNFR-alpha). Activating mutations of the RET proto-oncogene were found in multiple endocrine neoplasia (MEN) 2 and in sporadic medullary thyroid carcinoma and pheochromocytoma of neuroendocrine origin. Mutations of the RET proto-oncogene and the glial cell line-derived neurotrophic factor (GDNF) gene were examined in human pituitary tumors. No mutations of the RET proto-oncogene including the cysteine-rich region or codon 768 and 918 in the tyrosine kinase domain were detected in 172 human pituitary adenomas either by polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) or by PCR-restriction fragment length polymorphism (RFLP). Further, somatic mutations of the GDNF gene in 33 human pituitary adenomas were not detected by PCR-SSCP. One polymorphism of the GDNF gene at codon 145 of TGC or TGT was observed in a prolactinoma. The RET proto-oncogene message was detected in a normal human pituitary gland or 4 of 4 human pituitary adenomas with reverse transcription (RT)-PCR, and in rodent pituitary tumor cell lines with Western blotting. The expression of GDNF gene was detected in 1 of 4 human somatotroph adenomas, 1 of 2 corticotroph adenomas, and 2 of 6 rodent pituitary tumor cell lines with RT-PCR. Based on these, it is concluded that somatic mutations of the RET proto-oncogene or the GDNF gene do not appear to play a major role in the pituitary tumorigenesis in examined tumors.
|
| 巻・号 |
46(1)
|
| ページ |
199-207
|
| 公開日 |
1999-2-1
|
| DOI |
10.1507/endocrj.46.199
|
| PMID |
10426588
|
| MeSH |
Adenoma / genetics*
Animals
DNA Mutational Analysis
Drosophila Proteins*
Gene Expression
Glial Cell Line-Derived Neurotrophic Factor
Glial Cell Line-Derived Neurotrophic Factor Receptors
Humans
Mice
Mutation*
Nerve Growth Factors*
Nerve Tissue Proteins / genetics*
Pituitary Neoplasms / genetics*
Proto-Oncogene Mas
Proto-Oncogene Proteins / genetics*
Proto-Oncogene Proteins c-ret
RNA, Neoplasm / analysis
Rats
Receptor Protein-Tyrosine Kinases / genetics*
Reverse Transcriptase Polymerase Chain Reaction
|
| IF |
1.952
|
| リソース情報 |
| ヒト・動物細胞 |
MtT/S(RCB0528)
MtT/SM(RCB0530) |
