論文 - 詳細
RRC ID | 62209 |
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著者 | Chen DS, Chan KM. |
タイトル | PCR-cloning of tilapia ATP7A cDNA and its mRNA levels in tissues of tilapia following copper administrations. |
ジャーナル | Aquat Toxicol |
Abstract |
We are studying the toxicity of copper to tilapia and zebrafish and have found that the copper tolerance of tilapia and the sensitivity of zebrafish were due to several proteins' regulation mechanisms that were related to the effects of reactive oxygen species, mitochondrion copper transport, and stress response. To further reveal the mechanism of copper tolerance and sensitivity in tilapia and zebrafish, a full length cDNA of ATP7A was obtained in tilapia. Using real time quantitative PCR, the differential regulations of ATP7A in tilapia and zebrafish were studied. It was found that Cu(2+) gave a higher induction of ATP7A in tilapia than zebrafish, both in vivo and in vitro. These results suggest that the copper tolerance of tilapia may be due to higher expression level of ATP7A. |
巻・号 | 105(3-4) |
ページ | 717-27 |
公開日 | 2011-10-1 |
DOI | 10.1016/j.aquatox.2011.09.011 |
PII | S0166-445X(11)00259-1 |
PMID | 21996258 |
MeSH | Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism* Animals Base Sequence Cloning, Molecular Copper / toxicity* Copper-Transporting ATPases DNA, Complementary / chemistry Fish Proteins / genetics Fish Proteins / metabolism* Gene Expression Regulation / drug effects Molecular Sequence Data Phylogeny RNA, Messenger / metabolism Real-Time Polymerase Chain Reaction Sequence Analysis, DNA Tilapia / genetics Tilapia / metabolism* Water Pollutants, Chemical / toxicity* Zebrafish / genetics Zebrafish / metabolism Zebrafish Proteins / genetics Zebrafish Proteins / metabolism |
IF | 4.346 |
リソース情報 | |
ヒト・動物細胞 | Hepa-T1(RCB1156) |