| RRC ID |
62737
|
| 著者 |
Hu W, Zhang Y, Jiang Z, Wang L, Li J, Chen S, Dai N, Si J.
|
| タイトル |
The tumor promoting roles of erythropoietin/erythropoietin receptor signaling pathway in gastric cancer.
|
| ジャーナル |
Tumour Biol
|
| Abstract |
Erythropoietin (EPO), binding with its receptor (EPOR), plays an important role in erythropoiesis. EPOR is reported to be expressed in various non-hematopoietic cancers, including gastric cancer. Although recombinant human EPO (rhEPO) has been widely used clinically in anemia patients, it remains controversial whether it would promote tumor progression. In this study, we used siRNA interference method to downregulate EPOR expression to investigate the function of EPO/EPOR pathway in human gastric cancer cells. We found EPOR expressed significantly higher in gastric cancer tissues, and also in most gastric cancer cell lines. RhEPO promoted gastric cancer cell proliferation, migration in AGS cells, and promoted cells from G0/G1 stage to G2/M stage, but had no regulation on AGS cell apoptosis. Downregulation of EPOR expression by siRNA interference in AGS cells resulted in no significant effects on proliferation and invasiveness of the cells, but induced apoptosis (p < 0.05). Xenografted gastric tumor model was used to explore the effect of EPOR-overexpression on gastric cancer cells in vivo. Our result showed that overexpression of EPOR enhanced tumor formation in nude mice (p < 0.01). Our results suggest that EPO/EPOR pathway promotes gastric cancer formation, proliferation, migration, and decreases apoptosis.
|
| 巻・号 |
37(8)
|
| ページ |
11523-33
|
| 公開日 |
2016-8-1
|
| DOI |
10.1007/s13277-016-5053-7
|
| PII |
10.1007/s13277-016-5053-7
|
| PMID |
27086036
|
| MeSH |
Adult
Aged
Animals
Apoptosis / physiology
Blotting, Western
Erythropoietin / metabolism*
Female
Flow Cytometry
Gene Knockdown Techniques
Heterografts
Humans
Immunohistochemistry
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Middle Aged
Real-Time Polymerase Chain Reaction
Receptors, Erythropoietin / metabolism*
Signal Transduction / physiology
Stomach Neoplasms / pathology*
|
| IF |
3.65
|
| リソース情報 |
| ヒト・動物細胞 |
MKN45(RCB1001)
MKN28(RCB1000) |
