論文 - 詳細
| RRC ID | 63082 |
|---|---|
| 著者 | Minagawa Y, Ishino K, Wada R, Kudo M, Naito Z, Takeshita T, Ohashi R. |
| タイトル | High Expression of p21 as a Potential Therapeutic Target in Ovarian Clear-cell Carcinoma. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:DNA damage response (DDR), wherein p21 is a cell fate determinant, is a potential cancer therapeutic target. Molecular expression during DDR was explored in ovarian clear-cell carcinoma (CCC). MATERIALS AND METHODS:CHK1, CHK2, TP53 and p21 expression in DDR was examined using immunostaining in surgical sections of CCC (n=22). Molecular alterations in two types of CCC cell lines, JHOC-5 and JHOC-9, were investigated using western blot analysis. RESULTS:Expression of DDR-associated molecules was noted in most patients. While high p21 expression was found in half of the patients, the remaining patients exhibited low p21 expression. Treatment with UC2288, a p21 inhibitor, attenuated proliferation of both cell lines, more prominently in JHOC-9, resulting in reduced viability and subsequent apoptosis. CONCLUSION:p21 Inhibitor induced cell death in cells with high p21 expression, suggesting that p21 suppression can be a therapeutic strategy to treat patients with CCC. |
| 巻・号 | 40(10) |
| ページ | 5631-5639 |
| 公開日 | 2020-10-1 |
| DOI | 10.21873/anticanres.14576 |
| PII | 40/10/5631 |
| PMID | 32988887 |
| MeSH | Adult Aged Apoptosis / genetics Cell Line, Tumor Cell Proliferation / genetics Checkpoint Kinase 1 / genetics* Checkpoint Kinase 2 / genetics* Cyclin-Dependent Kinase Inhibitor p21 / genetics* DNA Damage / genetics Female Gene Expression Regulation, Neoplastic Humans Middle Aged Ovarian Neoplasms / genetics* Ovarian Neoplasms / pathology Tumor Suppressor Protein p53 / genetics* |
| IF | 1.994 |
| リソース情報 | |
| ヒト・動物細胞 | JHOC-5(RCB1520) JHOC-9(RCB2226) |