| RRC ID |
63102
|
| 著者 |
Kang JH, Katsikis G, Li Z, Sapp KM, Stockslager MA, Lim D, Vander Heiden MG, Yaffe MB, Manalis SR, Miettinen TP.
|
| タイトル |
Monitoring and modeling of lymphocytic leukemia cell bioenergetics reveals decreased ATP synthesis during cell division.
|
| ジャーナル |
Nat Commun
|
| Abstract |
The energetic demands of a cell are believed to increase during mitosis, but the rates of ATP synthesis and consumption during mitosis have not been quantified. Here, we monitor mitochondrial membrane potential of single lymphocytic leukemia cells and demonstrate that mitochondria hyperpolarize from the G2/M transition until the metaphase-anaphase transition. This hyperpolarization was dependent on cyclin-dependent kinase 1 (CDK1) activity. By using an electrical circuit model of mitochondria, we quantify mitochondrial ATP synthesis rates in mitosis from the single-cell time-dynamics of mitochondrial membrane potential. We find that mitochondrial ATP synthesis decreases by approximately 50% during early mitosis and increases back to G2 levels during cytokinesis. Consistently, ATP levels and ATP synthesis are lower in mitosis than in G2 in synchronized cell populations. Overall, our results provide insights into mitotic bioenergetics and suggest that cell division is not a highly energy demanding process.
|
| 巻・号 |
11(1)
|
| ページ |
4983
|
| 公開日 |
2020-10-5
|
| DOI |
10.1038/s41467-020-18769-y
|
| PII |
10.1038/s41467-020-18769-y
|
| PMID |
33020492
|
| PMC |
PMC7536222
|
| MeSH |
Adenosine Triphosphate / biosynthesis*
Animals
CDC2 Protein Kinase / metabolism
Cell Division*
Cell Line, Tumor
Cytokinesis
Energy Metabolism*
Mice
Mitochondria / metabolism
Mitosis
Models, Biological
|
| IF |
12.121
|
| リソース情報 |
| ヒト・動物細胞 |
Ba/F3(RCB4476) |
