論文 - 詳細
| RRC ID | 63225 |
|---|---|
| 著者 | Kaitsuka T, Tomizawa K. |
| タイトル | Generation of Functional Insulin-Producing Cells from Mouse Embryonic Stem Cells Through Protein Transduction of Transcription Factors. |
| ジャーナル | Methods Mol Biol |
| Abstract |
In this chapter, we describe a simple and unique method for the differentiation of mouse embryonic stem cells into insulin-producing cells. In addition to cytokines and growth factors, key transcription factors for pancreatic development are applied in this method through protein transduction technology. Furthermore, a combination of nanofiber plates and laminin coatings improves the yield of differentiated cells. The insulin-producing cells derived through this method express marker genes of mature β-cells and have an ability to secrete insulin; therefore, these cells are useful for fundamental studies on pancreatic development, drug development, and regenerative medicine for diabetes. |
| 巻・号 | 2211 |
| ページ | 85-96 |
| 公開日 | 2021-1-1 |
| DOI | 10.1007/978-1-0716-0943-9_7 |
| PMID | 33336272 |
| MeSH | Animals C-Peptide / biosynthesis Cell Differentiation / genetics* Cells, Cultured Fibroblasts / metabolism Gene Expression* Humans Insulin / biosynthesis Insulin-Secreting Cells / cytology Insulin-Secreting Cells / metabolism* Mice Mouse Embryonic Stem Cells / cytology* Mouse Embryonic Stem Cells / metabolism* Recombinant Proteins / genetics Recombinant Proteins / isolation & purification Transcription Factors / genetics* Transcription Factors / metabolism Transduction, Genetic* |
| リソース情報 | |
| ヒト・動物細胞 | E14tg2a(AES0135) |