| RRC ID |
63263
|
| 著者 |
Sekiya M, Kiyohara H, Maruyama H, Yabe T, Yamada H.
|
| タイトル |
Modulation of chemokine expression on intestinal epithelial cells by Kampo (traditional Japanese herbal) medicine, Hochuekkito, and its active ingredients.
|
| ジャーナル |
J Nat Med
|
| Abstract |
The intestinal epithelial cells sit at the interface between a lumen and a lamina propria or lymph nodes such as Peyer's patches, where they play important roles in maintaining intestinal homeostasis through chemokine secretion. This study investigated the effect of Hochuekkito (TJ-41)-a traditional Japanese herbal (Kampo) formula used as a tonic for weakness-on chemokine expression in intestinal epithelial cells in order to explore the mechanism of its modulating effect against mucosal immunity. When cells from the rat normal small intestinal epithelial cell-line IEC-6 were stimulated with TJ-41, mRNA expression of CC chemokine ligand (CCL) 11 (eotaxin), CCL20 (MIP-3α) and CCL25 (TECK) was enhanced. Oral administration of TJ-41 to methotrexate-treated mice enhanced mRNA expression of CCL25 and keratinocyte growth factor in the jejunum with, decreasing mRNA expression of the inflammatory marker tumor necrosis factor (TNF)-α. Although oral administration of TJ-41 did not affect CCL20 mRNA expression in villus epithelium of methotrexate-treated mice, enhancement of CCL20 mRNA expression was observed in Peyer's patches. Immunohistochemical analysis detected dense staining with anti-CCL20 antibody in the follicle-associated epithelium region of Peyer's patches in mice administered TJ-41. Analysis of active ingredients indicates that polysaccharide-containing macromolecules in TJ-41 contribute to the enhancement of CCL20 mRNA expression through an intracellular signal cascade via nuclear factor kappa B (NF-κB) activation.
|
| 巻・号 |
67(3)
|
| ページ |
626-35
|
| 公開日 |
2013-7-1
|
| DOI |
10.1007/s11418-012-0724-1
|
| PMID |
23203627
|
| MeSH |
Administration, Oral
Animals
Anti-Inflammatory Agents / administration & dosage
Anti-Inflammatory Agents / pharmacology*
Cell Line
Chemokine CCL11 / metabolism
Chemokine CCL20 / metabolism
Chemokines / genetics
Chemokines / metabolism*
Chemokines, CC / metabolism
Drugs, Chinese Herbal / administration & dosage
Drugs, Chinese Herbal / pharmacology*
Epithelial Cells / drug effects*
Epithelial Cells / immunology
Epithelial Cells / metabolism
Female
Fibroblast Growth Factor 7 / metabolism
Intestinal Mucosa / drug effects*
Intestinal Mucosa / immunology
Intestinal Mucosa / metabolism
Jejunum / drug effects*
Jejunum / immunology
Jejunum / metabolism
Medicine, Kampo*
Mice
Mice, Inbred BALB C
NF-kappa B / metabolism
Peyer's Patches / drug effects
Peyer's Patches / metabolism
Phytotherapy
Plants, Medicinal
RNA, Messenger / metabolism
Rats
Signal Transduction
Time Factors
Tumor Necrosis Factor-alpha / metabolism
|
| IF |
2.055
|
| リソース情報 |
| ヒト・動物細胞 |
IEC 6(RCB0993) |
