| RRC ID |
63283
|
| 著者 |
Lenis JA, Rico P, Ribelles JLG, Pacha-Olivenza MA, González-Martín ML, Bolívar FJ.
|
| タイトル |
Structure, morphology, adhesion and in vitro biological evaluation of antibacterial multi-layer HA-Ag/SiO2/TiN/Ti coatings obtained by RF magnetron sputtering for biomedical applications.
|
| ジャーナル |
Mater Sci Eng C Mater Biol Appl
|
| Abstract |
Biocompatible and antibacterial multi-layer coatings of hydroxyapatite (HA)-Ag/SiO2/TiN/Ti were obtained on the Ti-6Al-4V alloy, by means of the magnetron sputtering technique. During characterization of the coatings, the chemical composition was evaluated by energy dispersive X-ray spectroscopy and the phase analysis was carried out by X-ray diffraction. The morphology of the coatings was observed by field emission scanning electron microscopy, while transmission electron microscopy was used to appreciate their structure. The adhesion of the coatings to the substrate was evaluated by micro scratch test. The in vitro biological response was evaluated in terms of cytotoxicity, adhesion and differentiation of mouse mesenchymal stem cells, as well as adhesion and bacterial viability of Staphylococcus aureus strain. Through the compositional study carried out, the deposition of the HA phase was verified, with a Ca/P ratio close to 1.67 and the characteristic diffraction peaks of this compound. The structural study of the coatings evidenced the obtention of multi-layer architectures. The use of an intermediate SiO2/TiN/Ti trilayer was found to improve adhesion between HA-Ag and the substrate by 84%. Finally, the in vitro biological tests carried out indicated a potentially non-toxic character in the coatings. Additionally, an antibacterial effect was registered at low concentrations of Ag (<0.25 mg/L).
|
| 巻・号 |
116
|
| ページ |
111268
|
| 公開日 |
2020-11-1
|
| DOI |
10.1016/j.msec.2020.111268
|
| PII |
S0928-4931(20)31970-6
|
| PMID |
32806245
|
| MeSH |
Animals
Anti-Bacterial Agents / pharmacology
Coated Materials, Biocompatible / pharmacology
Durapatite*
Materials Testing
Mice
Microscopy, Electron, Scanning
Silicon Dioxide
Silver / pharmacology
Tin
Titanium* / pharmacology
X-Ray Diffraction
|
| IF |
5.88
|
| リソース情報 |
| ヒト・動物細胞 |
10T1/2(RCB0247) |
