RRC ID |
63913
|
著者 |
Chung SY, Seki H, Fujisawa Y, Shimoda Y, Hiraga S, Nomura Y, Saito K, Ishimoto M, Muranaka T.
|
タイトル |
A cellulose synthase-derived enzyme catalyses 3-O-glucuronosylation in saponin biosynthesis.
|
ジャーナル |
Nat Commun
|
Abstract |
Triterpenoid saponins are specialised metabolites distributed widely in the plant kingdom that consist of one or more sugar moieties attached to triterpenoid aglycones. Despite the widely accepted view that glycosylation is catalysed by UDP-dependent glycosyltransferase (UGT), the UGT which catalyses the transfer of the conserved glucuronic acid moiety at the C-3 position of glycyrrhizin and various soyasaponins has not been determined. Here, we report that a cellulose synthase superfamily-derived glycosyltransferase (CSyGT) catalyses 3-O-glucuronosylation of triterpenoid aglycones. Gene co-expression analyses of three legume species (Glycyrrhiza uralensis, Glycine max, and Lotus japonicus) reveal the involvement of CSyGTs in saponin biosynthesis, and we characterise CSyGTs in vivo using Saccharomyces cerevisiae. CSyGT mutants of L. japonicus do not accumulate soyasaponin, but the ectopic expression of endoplasmic reticulum membrane–localised CSyGTs in a L. japonicus mutant background successfully complement soyasaponin biosynthesis. Finally, we produced glycyrrhizin de novo in yeast, paving the way for sustainable production of high-value saponins.
|
巻・号 |
11(1)
|
ページ |
5664
|
公開日 |
2020-11-16
|
DOI |
10.1038/s41467-020-19399-0
|
PII |
10.1038/s41467-020-19399-0
|
PMID |
33199711
|
PMC |
PMC7669905
|
MeSH |
Biocatalysis*
Biosynthetic Pathways
Endoplasmic Reticulum / metabolism
Gene Expression Regulation, Plant
Glucosyltransferases / metabolism*
Glucuronic Acid / metabolism*
Glycosylation
Glycyrrhiza uralensis / genetics
Glycyrrhizic Acid / metabolism
Likelihood Functions
Lotus / genetics
Phylogeny
Saccharomyces cerevisiae / metabolism
Saponins / biosynthesis*
Saponins / chemistry
Soybeans / genetics
Substrate Specificity
Triterpenes / metabolism
Uridine Diphosphate Glucuronic Acid / metabolism
|
IF |
12.121
|
リソース情報 |
シロイヌナズナ / 植物培養細胞・遺伝子 |
pda05996 |