論文 - 詳細
RRC ID | 6589 |
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著者 | Monira P, Koyama Y, Fukutomi R, Yasui K, Isemura M, Yokogoshi H. |
タイトル | Effects of Japanese mistletoe lectin on cytokine gene expression in human colonic carcinoma cells and in the mouse intestine. |
ジャーナル | Biomed Res |
Abstract |
Mistletoe lectins have various biological activities including anti-cancer and immunomodulatory effects. We previously isolated a lectin (ML-J) from Japanese mistletoe. In the present study, we examined the effects of ML-J on cytokine gene expression in human colon adenocarcinoma Caco-2 cells and in the mouse intestine. The results of reverse transcription-polymerase chain reaction and quantitative real-time polymerase chain reaction indicated that ML-J caused an upregulation of the gene expression of the proinflammatory cytokines interleukin (IL)-8, tumor necrosis factor-alpha (TNF-alpha) and IL-6 in Caco-2 cells and TNF-alpha and IL-6 in the duodenum. This study provides the first example to show that a perorally administered plant lectin affects gene expression in the duodenum. |
巻・号 | 30(5) |
ページ | 303-9 |
公開日 | 2009-10-1 |
DOI | 10.2220/biomedres.30.303 |
PII | JST.JSTAGE/biomedres/30.303 |
PMID | 19887727 |
MeSH | Animals Caco-2 Cells / drug effects* Caco-2 Cells / physiology Cytokines* / genetics Cytokines* / metabolism Gene Expression Regulation / drug effects* Humans Intestines / anatomy & histology Intestines / drug effects* Intestines / physiology Mice Mistletoe / chemistry* Plant Lectins / pharmacology* |
IF | 1.429 |
引用数 | 7 |
WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL |
リソース情報 | |
ヒト・動物細胞 | CACO-2(RCB0988) |