| RRC ID |
660
|
| 著者 |
Hong Z, Ueguchi-Tanaka M, Umemura K, Uozu S, Fujioka S, Takatsuto S, Yoshida S, Ashikari M, Kitano H, Matsuoka M.
|
| タイトル |
A rice brassinosteroid-deficient mutant, ebisu dwarf (d2), is caused by a loss of function of a new member of cytochrome P450.
|
| ジャーナル |
Plant Cell
|
| Abstract |
We characterized a rice dwarf mutant, ebisu dwarf (d2). It showed the pleiotropic abnormal phenotype similar to that of the rice brassinosteroid (BR)-insensitive mutant, d61. The dwarf phenotype of d2 was rescued by exogenous brassinolide treatment. The accumulation profile of BR intermediates in the d2 mutants confirmed that these plants are deficient in late BR biosynthesis. We cloned the D2 gene by map-based cloning. The D2 gene encoded a novel cytochrome P450 classified in CYP90D that is highly similar to the reported BR synthesis enzymes. Introduction of the wild D2 gene into d2-1 rescued the abnormal phenotype of the mutants. In feeding experiments, 3-dehydro-6-deoxoteasterone, 3-dehydroteasterone, and brassinolide effectively caused the lamina joints of the d2 plants to bend, whereas more upstream compounds did not cause bending. Based on these results, we conclude that D2/CYP90D2 catalyzes the steps from 6-deoxoteasterone to 3-dehydro-6-deoxoteasterone and from teasterone to 3-dehydroteasterone in the late BR biosynthesis pathway.
|
| 巻・号 |
15(12)
|
| ページ |
2900-10
|
| 公開日 |
2003-12-1
|
| DOI |
10.1105/tpc.014712
|
| PII |
tpc.014712
|
| PMID |
14615594
|
| PMC |
PMC282825
|
| MeSH |
Amino Acid Sequence
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism
Brassinosteroids
Cholestanols / metabolism*
Cholestanols / pharmacology
Cytochrome P-450 Enzyme System / genetics
Cytochrome P-450 Enzyme System / metabolism*
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Molecular Sequence Data
Mutation
Oryza / drug effects
Oryza / genetics
Oryza / metabolism*
Phenotype
Phylogeny
Physical Chromosome Mapping
Plant Growth Regulators / metabolism*
Plant Growth Regulators / pharmacology
Plant Proteins / genetics*
Plant Proteins / metabolism
Sequence Homology, Amino Acid
Steroids, Heterocyclic / metabolism*
Steroids, Heterocyclic / pharmacology
|
| IF |
9.618
|
| 引用数 |
323
|
|
WOS 分野
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| イネ |
d2-2: Ebis dwarf (九大のT65のNILが使用されている）
この論文に関連して
d61−１：TCM347
TCM348、（TCM347とTCM348は、クローンと考えられる）、d61−4：odm24（胚発生の突然変異体から同定された）
d11−３：TCM2410 |
