論文 - 詳細
RRC ID | 6604 |
---|---|
著者 | Yamaoka K, Okayama Y, Kaminuma O, Katayama K, Mori A, Tatsumi H, Nemoto S, Hiroi T. |
タイトル | Proteomic Approach to FcepsilonRI aggregation-initiated signal transduction cascade in human mast cells. |
ジャーナル | Int Arch Allergy Immunol |
Abstract |
BACKGROUND:Mast cells (MCs) play a central role in allergic reactions through high-affinity IgE receptor (FcepsilonRI)-mediated responses. Many attempts have been performed to investigate MC functions, though molecular bases of the intracellular signaling cascade through FcepsilonRI, especially in human MCs, remain scant and unexplored. METHODS:Human MCs were differentiated from CD34+ cells by culture with stem cell factor, IL-6 and IL-3. The differential phosphorylation profiles of protein tyrosine residues in the resulting MCs with or without FcepsilonRI aggregation were examined by two-dimensional gel electrophoresis. The candidate phosphoproteins of interest were picked, in-gel digested and mass spectrometry fingerprinted. RESULTS:Approximately 40 proteins in MCs were phosphorylated on their tyrosine residues in response to activation and some of them were identified. Particularly IL-31 receptor alpha, solute carrier family 39, syntaxin 5 and heterogeneous nuclear ribonucleoprotein are newly identified as phosphoproteins that are potentially involved in the MC signaling cascade through FcepsilonRI. CONCLUSION:Our present phosphoproteome data may provide the clue to understand the molecular mechanisms for the activation of human MCs. |
巻・号 | 149 Suppl 1 |
ページ | 73-6 |
公開日 | 2009-1-1 |
DOI | 10.1159/000211376 |
PII | 000211376 |
PMID | 19494509 |
MeSH | Cation Transport Proteins / metabolism Heterogeneous-Nuclear Ribonucleoproteins / metabolism Humans Mast Cells / metabolism* Phosphoproteins / metabolism* Phosphorylation Proteomics Qa-SNARE Proteins / metabolism Receptors, IgE / metabolism* Receptors, Interleukin / metabolism Signal Transduction* Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tyrosine / metabolism* |
IF | 2.917 |
引用数 | 16 |
WOS 分野 | ALLERGY IMMUNOLOGY |
リソース情報 | |
ヒト・動物細胞 |