| RRC ID |
66061
|
| 著者 |
Xie ME, Adam Y, Fan LZ, Böhm UL, Kinsella I, Zhou D, Rozsa M, Singh A, Svoboda K, Paninski L, Cohen AE.
|
| タイトル |
High-fidelity estimates of spikes and subthreshold waveforms from 1-photon voltage imaging in vivo.
|
| ジャーナル |
Cell Rep
|
| Abstract |
The ability to probe the membrane potential of multiple genetically defined neurons simultaneously would have a profound impact on neuroscience research. Genetically encoded voltage indicators are a promising tool for this purpose, and recent developments have achieved a high signal-to-noise ratio in vivo with 1-photon fluorescence imaging. However, these recordings exhibit several sources of noise and signal extraction remains a challenge. We present an improved signal extraction pipeline, spike-guided penalized matrix decomposition-nonnegative matrix factorization (SGPMD-NMF), which resolves supra- and subthreshold voltages in vivo. The method incorporates biophysical and optical constraints. We validate the pipeline with simultaneous patch-clamp and optical recordings from mouse layer 1 in vivo and with simulated and composite datasets with realistic noise. We demonstrate applications to mouse hippocampus expressing paQuasAr3-s or SomArchon1, mouse cortex expressing SomArchon1 or Voltron, and zebrafish spines expressing zArchon1.
|
| 巻・号 |
35(1)
|
| ページ |
108954
|
| 公開日 |
2021-4-6
|
| DOI |
10.1016/j.celrep.2021.108954
|
| PII |
S2211-1247(21)00268-0
|
| PMID |
33826882
|
| PMC |
PMC8095336
|
| MeSH |
Action Potentials / physiology*
Algorithms
Animals
Computer Simulation
Hippocampus / physiology
Imaging, Three-Dimensional*
Mice, Transgenic
Photons*
Pyramidal Cells / physiology
Reproducibility of Results
Signal Transduction
Zebrafish
|
| IF |
8.109
|
| リソース情報 |
| ゼブラフィッシュ |
Tg(vglut2a:Gal4) |
