| RRC ID |
67125
|
| 著者 |
Wakabayashi R, Sakuragi M, Kozaka S, Tahara Y, Kamiya N, Goto M.
|
| タイトル |
Solid-in-Oil Peptide Nanocarriers for Transcutaneous Cancer Vaccine Delivery against Melanoma.
|
| ジャーナル |
Mol Pharm
|
| Abstract |
Cancer vaccines represent a prophylactic or therapeutic method of suppressing cancer by activating the adaptive immune system. The immune response is initiated by the delivery of tumor antigens to antigen presenting cells (APCs). The use of peptides as vaccine antigens is advantageous, especially in the availability and productivity of pure and defined antigens. However, their limited immunogenicity remains a major drawback, and therefore, the utilization of nanocarriers as a means of delivering antigens to target cells and/or the addition of immune stimulants have been investigated as an efficient peptide-based cancer vaccine. We have developed a solid-in-oil (S/O) nanodispersion as a transcutaneous nanocarrier for hydrophilic molecules. This system has attractive features as a peptide nanocarrier for cancer vaccines, including transcutaneous targeting of professional APCs in the skin, high encapsulation efficacy of hydrophilic molecules, and capacity for coloading with a variety of immune stimulants such as adjuvants. We therefore sought to utilize the developed S/O nanodispersion for the delivery of the tyrosine-related protein 2 peptide, TRP-2180-188, as a peptide antigen against melanoma. Transcutaneous vaccination of the S/O nanodispersion coloaded with adjuvant R-848 was associated with a significant inhibition of melanoma growth and suppression of lung metastasis in tumor-bearing mice. Our findings indicate the potential of S/O nanodispersions as an endogenous peptide carrier for cancer vaccines.
|
| 巻・号 |
15(3)
|
| ページ |
955-961
|
| 公開日 |
2018-3-5
|
| DOI |
10.1021/acs.molpharmaceut.7b00894
|
| PMID |
29397746
|
| MeSH |
Adjuvants, Immunologic / administration & dosage
Administration, Cutaneous
Animals
Antigens, Neoplasm / administration & dosage
Antigens, Neoplasm / chemistry
Antigens, Neoplasm / immunology
Cancer Vaccines / administration & dosage*
Cancer Vaccines / immunology
Cell Line, Tumor / transplantation
Drug Carriers / chemistry*
Drug Compounding / methods
Female
Humans
Hydrophobic and Hydrophilic Interactions
Imidazoles / administration & dosage
Immunogenicity, Vaccine
Melanoma, Experimental / immunology
Melanoma, Experimental / therapy*
Membrane Proteins / administration & dosage*
Membrane Proteins / chemistry
Membrane Proteins / immunology
Mice
Mice, Inbred C57BL
Nanoparticles / chemistry
Oils / chemistry
Peptide Fragments / administration & dosage*
Peptide Fragments / chemistry
Peptide Fragments / immunology
Skin Neoplasms / immunology
Skin Neoplasms / therapy*
Treatment Outcome
|
| IF |
4.321
|
| リソース情報 |
| ヒト・動物細胞 |
B16F10(RCB2630) |
