| RRC ID |
67184
|
| 著者 |
Takahashi N, Aoyama F, Hiyoshi M, Kataoka H, Sawaguchi A.
|
| タイトル |
Establishment and biological characterization of a novel cell line derived from hepatoid adenocarcinoma originated at the ampulla of Vater.
|
| ジャーナル |
Int J Oncol
|
| Abstract |
Hepatoid adenocarcinoma is a rare gastrointestinal tumor and mostly reported in the stomach. Effective chemotherapy has yet to be developed to improve poor prognosis. The present study was undertaken to establish a useful cell line derived from a hepatoid adenocarcinoma, possibly leading to a new therapeutic strategy. The new human cell line VAT-39 was established from a metastatic lymph node of a 69-year-old Japanese male patient with hepatoid adenocarcinoma of the ampulla of Vater. The primary tumor and metastatic lymph node were composed of hepatoid adenocarcinoma cells exhibiting immunohistochemical reactivity for alpha-fetoprotein (AFP) and glypican-3 (GPC3). In the metastatic lymph node, Periodic acid-Schiff (PAS) staining clarified diffuse deposition of glycogen in the cytoplasm, indicating analogous characteristics to the primary hepatoid adenocarcinoma. Moreover, VAT-39 cells produced high levels of AFP in the cultured medium, and reverse-transcriptase polymerase chain reaction (RT-PCR) verified increased expression of GPC3 mRNA in this cell line. Further, we evaluated the sensitivity to major chemotherapeutic drugs against the bile duct cancer. Neither 5-fluorouracil nor gemcitabine showed particular sensitivity to this cell line. The tumorigenicity of the cultured cells was confirmed in athymic nude mice and the histological features of the explanted tumor were similar to the VAT-39 cell line. The present VAT-39 is the first hepatoid adenocarcinoma cell line that originates from the ampulla of Vater and it will be applicable for basic biological studies searching for new strategies of molecular targeted chemotherapy to this disease.
|
| 巻・号 |
44(4)
|
| ページ |
1139-45
|
| 公開日 |
2014-4-1
|
| DOI |
10.3892/ijo.2014.2282
|
| PMID |
24481592
|
| MeSH |
Adenocarcinoma / drug therapy
Adenocarcinoma / pathology*
Aged
Ampulla of Vater / cytology
Ampulla of Vater / pathology*
Animals
Antimetabolites, Antineoplastic / pharmacology
Bile Duct Neoplasms / drug therapy
Bile Duct Neoplasms / pathology*
Deoxycytidine / analogs & derivatives
Deoxycytidine / pharmacology
Drug Resistance, Neoplasm
Fluorouracil / pharmacology
Gastrointestinal Neoplasms / drug therapy
Gastrointestinal Neoplasms / pathology*
Gemcitabine
Glycogen / metabolism
Glypicans / genetics
Glypicans / immunology
Humans
Lymph Nodes / cytology
Lymph Nodes / pathology*
Lymphatic Metastasis
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasm Transplantation
RNA, Messenger / biosynthesis
Transplantation, Heterologous
Tumor Cells, Cultured
alpha-Fetoproteins / biosynthesis
alpha-Fetoproteins / immunology
|
| IF |
3.899
|
| リソース情報 |
| ヒト・動物細胞 |
HuCCT1(RCB1960)
HuH-28(RCB1943)
TFK-1(RCB2537)
TKKK(RCB1907)
TGBC2TKB(RCB1130)
TGBC14TKB(RCB1186)
TGBC18TKB(RCB1169) |
