論文 - 詳細
| RRC ID | 67671 |
|---|---|
| 著者 | Kobayashi H, Yamashita Y, Iwase A, Yoshikawa Y, Yasui H, Kawai Y, Uchida K, Uno N, Akatsuka S, Takahashi T, Kikkawa F, Toyokuni S. |
| タイトル | The ferroimmunomodulatory role of ectopic endometriotic stromal cells in ovarian endometriosis. |
| ジャーナル | Fertil Steril |
| Abstract |
OBJECTIVE:To understand the role of ectopic endometriotic stromal cells in ovarian endometriosis (OEM) and the associated risks for infertility and carcinogenesis. DESIGN:Analyses of secreted proteins and gene expression using immortalized eutopic/ectopic endometrial(-otic) stromal cells from OEM. SETTING:University. PATIENT(S):Women with and without OEM. INTERVENTION(S):Samples of endometrial(-otic) tissue from women with or without OEM. MAIN OUTCOME MEASURE(S):Immunohistochemical analysis of oxidative stress in OEM, gene expression profiles, and the identification of secreted proteins by mass spectrometry in immortalized endometrial(-otic) stromal cells. RESULT(S):4-Hydroxy-2-nonenal-modified proteins and carboxymethyllysine were abundant in the stroma, rather than epithelia, of OEM patients, indicating the presence of oxidative stress. Immortalized ectopic endometriotic stromal cells exhibited high IRP1/IRP2/HIF-1β expression and contained lower amounts of iron and copper than their eutopic counterparts. Expression profiles, in combination with protein identification, revealed that complement component 3 (C3) and pentraxin-3 (PTX3) are the major proteins secreted from immortalized ectopic endometriotic stromal cells. Complement-3/PTX3 promoted the secretion of various cytokines by THP1 macrophage cells and thus supported M1 differentiation. CONCLUSION(S):Immortalized ectopic endometriotic stromal cells in OEM predominantly secrete C3 and PTX3 and exhibit a differential regulation of iron metabolism. |
| 巻・号 | 98(2) |
| ページ | 415-22.e1-12 |
| 公開日 | 2012-8-1 |
| DOI | 10.1016/j.fertnstert.2012.04.047 |
| PII | S0015-0282(12)00515-8 |
| PMID | 22633261 |
| MeSH | C-Reactive Protein / physiology Cell Line, Transformed Cells, Cultured Choristoma / metabolism* Choristoma / pathology Complement C3 / physiology Endometriosis / metabolism* Endometriosis / pathology Endometrium* / cytology Endometrium* / immunology Endometrium* / metabolism Female Humans Immunomodulation / physiology* Iron / immunology Iron / metabolism* Ovary / immunology Ovary / metabolism* Ovary / pathology Serum Amyloid P-Component / physiology Stromal Cells / metabolism |
| IF | 6.312 |
| リソース情報 | |
| 遺伝子材料 | |
| ヒト・動物細胞 | THP-1(RCB1189) |