Reference - Detail
| RRC ID | 67690 |
|---|---|
| Author | Su S, Phua SC, DeRose R, Chiba S, Narita K, Kalugin PN, Katada T, Kontani K, Takeda S, Inoue T. |
| Title | Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia. |
| Journal | Nat Methods |
| Abstract |
Visualization of signal transduction in live primary cilia constitutes a technical challenge owing to the organelle's submicrometer dimensions and close proximity to the cell body. Using a genetically encoded calcium indicator targeted to primary cilia, we visualized calcium signaling in cilia of mouse fibroblasts and kidney cells upon chemical or mechanical stimulation with high specificity, high sensitivity and wide dynamic range. |
| Volume | 10(11) |
| Pages | 1105-7 |
| Published | 2013-11-1 |
| DOI | 10.1038/nmeth.2647 |
| PII | nmeth.2647 |
| PMID | 24056873 |
| PMC | PMC3860264 |
| MeSH | Animals Calcium Signaling / genetics* Cilia / metabolism* Mice Signal Transduction |
| IF | 30.822 |
| Resource | |
| DNA material | CSII-CMV-MCS-IRES2-Bsd (RDB04385) CS-CDF-CG-PRE (RDB04379) |