RRC ID |
67852
|
著者 |
Thongsiri C, Nagai-Yoshioka Y, Yamasaki R, Adachi Y, Usui M, Nakashima K, Nishihara T, Ariyoshi W.
|
タイトル |
Schizophyllum commune β-glucan: Effect on interleukin-10 expression induced by lipopolysaccharide from periodontopathic bacteria.
|
ジャーナル |
Carbohydr Polym
|
Abstract |
β-glucans are potent immunomodulators, with effects on innate and adaptive immune responses via dectin-1 as the main receptor. In this study, we investigated the biological effect of β-glucan from Schizophyllum commune, called Schizophyllan (SPG) on Interleukin-10 (IL-10) expression induced by a lipopolysaccharide (LPS) from Aggregatibacter actinomycetemcomitans in murine macrophages (J774.1). SPG and dectin-1 interaction up-regulates LPS-induced IL-10 expression. The regulative effect of SPG on IL-10 expression is dependent on prolongation of nuclear translocation activity of nuclear factor-kappa B (NF-κBα) pathway induced by LPS. We also found that LPS-induced phosphorylation of mitogen- and stress-activated protein kinase 1 (MSK1) and cAMP-responsive-element-binding protein (CREB), followed by up-regulation of IL-10, was stimulated by SPG priming via activation of the spleen tyrosine kinase (Syk). Our data indicate that SPG augments the anti-inflammatory response in murine macrophages which can be useful to create an intervention for periodontal disease treatment.
|
巻・号 |
253
|
ページ |
117285
|
公開日 |
2021-2-1
|
DOI |
10.1016/j.carbpol.2020.117285
|
PII |
S0144-8617(20)31458-2
|
PMID |
33278951
|
MeSH |
Adjuvants, Immunologic / metabolism
Adjuvants, Immunologic / pharmacology*
Aggregatibacter actinomycetemcomitans / chemistry*
Animals
Fungal Polysaccharides / metabolism
Fungal Polysaccharides / pharmacology*
Interleukin-10 / metabolism*
Lectins, C-Type / metabolism
Lipopolysaccharides / pharmacology*
Macrophages / drug effects
Macrophages / immunology*
Mice
NF-kappa B / metabolism
Pasteurellaceae Infections / drug therapy
Pasteurellaceae Infections / microbiology
Periodontal Diseases / drug therapy
Periodontal Diseases / microbiology
Phosphorylation / drug effects
Schizophyllum / chemistry*
Signal Transduction / drug effects
Sizofiran / metabolism
Sizofiran / pharmacology*
|
IF |
7.182
|
リソース情報 |
ヒト・動物細胞 |
J774.1(RCB0434) |