| RRC ID |
68885
|
| 著者 |
Kanadome T, Hoshino N, Nagai T, Matsuda T, Yagi T.
|
| タイトル |
Development of FRET-based indicators for visualizing homophilic trans interaction of a clustered protocadherin.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Clustered protocadherins (Pcdhs), which are cell adhesion molecules, play a fundamental role in self-recognition and non-self-discrimination by conferring diversity on the cell surface. Although systematic cell-based aggregation assays provide information regarding the binding properties of Pcdhs, direct visualization of Pcdh trans interactions across cells remains challenging. Here, we present Förster resonance energy transfer (FRET)-based indicators for directly visualizing Pcdh trans interactions. We developed the indicators by individually inserting FRET donor and acceptor fluorescent proteins (FPs) into the ectodomain of Pcdh molecules. They enabled successful visualization of specific trans interactions of Pcdh and revealed that the Pcdh trans interaction is highly sensitive to changes in extracellular Ca2+ levels. We expect that FRET-based indicators for visualizing Pcdh trans interactions will provide a new approach for investigating the roles of Pcdh in self-recognition and non-self-discrimination processes.
|
| 巻・号 |
11(1)
|
| ページ |
22237
|
| 公開日 |
2021-11-15
|
| DOI |
10.1038/s41598-021-01481-2
|
| PII |
10.1038/s41598-021-01481-2
|
| PMID |
34782670
|
| PMC |
PMC8593154
|
| MeSH |
Cell Line
Fluorescence Resonance Energy Transfer / methods*
Humans
Molecular Imaging / methods*
Protein Binding
Protein Interaction Domains and Motifs
Protein Multimerization
Protein Transport
Protocadherins / metabolism*
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
293T(RCB2202)
K562(RCB1897) |
