論文 - 詳細
| RRC ID | 69064 |
|---|---|
| 著者 | Tanaka N, Minemura C, Asai S, Kikkawa N, Kinoshita T, Oshima S, Koma A, Kasamatsu A, Hanazawa T, Uzawa K, Seki N. |
| タイトル | Identification of miR-199-5p and miR-199-3p Target Genes: Paxillin Facilities Cancer Cell Aggressiveness in Head and Neck Squamous Cell Carcinoma. |
| ジャーナル | Genes (Basel) |
| Abstract |
Our previous study revealed that the miR-199 family (miR-199a-5p/-3p and miR-199b-5p/-3p) acts as tumor-suppressive miRNAs in head and neck squamous cell carcinoma (HNSCC). Furthermore, recent studies have indicated that the passenger strands of miRNAs are involved in cancer pathogenesis. The aim of this study was to identify cancer-promoting genes commonly regulated by miR-199-5p and miR-199-3p in HNSCC cells. Our in silico analysis and luciferase reporter assay identified paxillin (PXN) as a direct target of both miR-199-5p and miR-199-3p in HNSCC cells. Analysis of the cancer genome atlas (TCGA) database showed that expression of PXN significantly predicted a worse prognosis (5-year overall survival rate; p = 0.0283). PXN expression was identified as an independent factor predicting patient survival according to multivariate Cox regression analyses (p = 0.0452). Overexpression of PXN was detected in HNSCC clinical specimens by immunostaining. Functional assays in HNSCC cells showed that knockdown of PXN expression attenuated cancer cell migration and invasion, suggesting that aberrant expression of PXN contributed to HNSCC cell aggressiveness. Our miRNA-based approach will provide new insights into the molecular pathogenesis of HNSCC. |
| 巻・号 | 12(12) |
| 公開日 | 2021-11-27 |
| DOI | 10.3390/genes12121910 |
| PII | genes12121910 |
| PMID | 34946859 |
| PMC | PMC8701835 |
| MeSH | Cell Line, Tumor Cell Movement Cell Proliferation Computational Biology / methods Databases, Genetic Gene Expression Regulation, Neoplastic* Head and Neck Neoplasms / genetics Head and Neck Neoplasms / metabolism Head and Neck Neoplasms / pathology* Humans MicroRNAs / genetics* Neoplasm Grading Paxillin / genetics Paxillin / metabolism* Squamous Cell Carcinoma of Head and Neck / genetics Squamous Cell Carcinoma of Head and Neck / metabolism Squamous Cell Carcinoma of Head and Neck / pathology* Survival Rate |
| IF | 3.759 |
| リソース情報 | |
| ヒト・動物細胞 | SAS(RCB1974) Sa3(RCB0980) |