| RRC ID |
71545
|
| 著者 |
Sun J, Li X, Qiu Y, Xue X, Zhang M, Yang W, Zhou D, Hu L, Lu R, Zhang Y.
|
| タイトル |
Quorum sensing regulates transcription of the pilin gene mshA1 of MSHA pilus in Vibrio parahaemolyticus.
|
| ジャーナル |
Gene
|
| Abstract |
Vibrio parahaemolyticus produces two types of IV pili: mannose-sensitive haemagglutinin type IV pili (MSHA) and chitin-regulated pili (ChiRP). Both of them are required for biofilm formation and the pathogen persistence in hosts. However, there are few reports on the regulation of their expression. In the present study, we showed that the master quorum sensing (QS) regulators AphA and OpaR oppositely regulated the transcription of mshA1 encoding the pilin of MSHA pilus in V. parahaemolyticus. At low cell density (LCD), AphA indirectly repressed mshA1 transcription. In contrast, at high cell density (HCD), OpaR bound to the regulatory DNA region of mshA1 to activate its transcription. Oppositely regulation of mshA1 by AphA and OpaR led to a gradual increase in the expression level of mshA1 from LCD to HCD. Thus, regulation of type IV pili production was one of the mechanisms that V. parahaemolyticus adopted to control biofilm formation.
|
| 巻・号 |
807
|
| ページ |
145961
|
| 公開日 |
2022-1-10
|
| DOI |
10.1016/j.gene.2021.145961
|
| PII |
S0378-1119(21)00556-4
|
| PMID |
34530088
|
| MeSH |
Bacterial Proteins / genetics
Biofilms / growth & development
Cell Count
Fimbriae Proteins / genetics*
Fimbriae Proteins / metabolism
Gene Expression / genetics
Gene Expression Regulation, Bacterial / genetics
Mannose-Binding Lectin / genetics
Mannose-Binding Lectin / metabolism
Quorum Sensing / genetics*
Trans-Activators / genetics
Trans-Activators / metabolism
Transcription Factors / genetics
Transcription Factors / metabolism
Vibrio / genetics*
Vibrio / metabolism
Vibrio cholerae / genetics
Vibrio cholerae / metabolism
Vibrio parahaemolyticus / genetics
Vibrio parahaemolyticus / metabolism
|
| IF |
2.984
|
| リソース情報 |
| 病原細菌 |
V. parahaemolyticus RIMD 2210633 |
