論文 - 詳細
| RRC ID | 72221 |
|---|---|
| 著者 | Takaki W, Konishi H, Shoda K, Arita T, Kataoka S, Shibamoto J, Furuke H, Takabatake K, Shimizu H, Komatsu S, Shiozaki A, Fujiwara H, Masuda K, Otsuji E. |
| タイトル | Significance of Circular FAT1 as a Prognostic Factor and Tumor Suppressor for Esophageal Squamous Cell Carcinoma. |
| ジャーナル | Ann Surg Oncol |
| Abstract |
BACKGROUND:Circular RNA is a novel endogenous non-coding RNA with a stable loop structure, and theories for its biogenesis and usefulness as a biomarker in various cancers have been proposed. The present study investigated the significance of circular FAT1 (circFAT1) as a novel biomarker in esophageal squamous cell carcinoma (ESCC). METHOD:CircFAT1 expression levels were measured in ESCC cell lines and the effects of downregulating circFAT1 on cell migration and invasion were examined using a transwell assay. The functions of miR-548g, which will be sponged by circFAT1, were assessed. Furthermore, the expression of circFAT1 was evaluated in 51 radically resected ESCC tissue samples using quantitative reverse transcription polymerase chain reaction (qRT-PCR). The relationships between circFAT1 expression, clinicopathological factors, and patient prognosis were analyzed. RESULTS:CircFAT1 expression levels were significantly lower in tumor tissue than in adjacent non-tumorous mucosal tissue (p = 0.01). The downregulation of circFAT1 expression promoted ESCC cell migration and invasive ability, but not proliferation. The expression of miR-548g was upregulated by the downregulation of circFAT1. The overexpression of miR-548g also promoted ESCC cell migration and invasion. Recurrence-free survival (p = 0.02) and cancer-specific survival (p = 0.04) rates were significantly higher in patients with elevated circFAT1 expression levels. CONCLUSION:The expression level of circFAT1 is a novel prognostic marker in ESCC patients. New treatment strategies may be developed using the tumor suppressive functions of circFAT1. |
| 巻・号 | 28(13) |
| ページ | 8508-8518 |
| 公開日 | 2021-12-1 |
| DOI | 10.1245/s10434-021-10089-9 |
| PII | 10.1245/s10434-021-10089-9 |
| PMID | 34185205 |
| PMC | PMC8591040 |
| MeSH | Cadherins / genetics Cell Line, Tumor Cell Movement Cell Proliferation Esophageal Neoplasms* / genetics Esophageal Squamous Cell Carcinoma* / genetics Gene Expression Regulation, Neoplastic Humans MicroRNAs* / genetics Neoplasm Invasiveness Prognosis |
| IF | 4.061 |
| リソース情報 | |
| ヒト・動物細胞 | TE-5(RCB1949) TE-8(RCB2098) TE-11(RCB2100) TE-15(RCB1951) WI-38(RCB0702) |