| RRC ID |
72589
|
| 著者 |
Yeo SY, Ha SY, Yu EJ, Lee KW, Kim JH, Kim SH.
|
| タイトル |
ZNF282 (Zinc finger protein 282), a novel E2F1 co-activator, promotes esophageal squamous cell carcinoma.
|
| ジャーナル |
Oncotarget
|
| Abstract |
Zinc finger protein 282 (ZNF282) is a newly identified transcription factor and little is known about its expression and function. Originally, ZNF282 is known to bind U5RE (U5 repressive element) of HLTV-1 (human T cell leukemia virus type 1) with a repressive effect. Recently we reported that ZNF282 functions as an estrogen receptor co-activator and plays an essential role in breast tumorigenesis. Although these results suggest the possible role of ZNF282 in cancers, clinical significance and function of ZNF282 are completely unknown in most of cancers. Here we found that ZNF282 was frequently overexpressed in esophageal squamous cell carcinoma (ESCC) (n=165) compared with normal esophageal epithelium and its overexpression was correlated with adverse clinical outcome. Multivariate survival analysis indicated that ZNF282 expression was an independent prognostic predictor for poor survival in ESCC (HR: 2.56 (95% CI 1.54-4.26), p<0.001). In addition, depletion of ZNF282 inhibited the cell cycle progression, migration, and invasion of ESCC cells and reduced the tumorigenicity of ESCC xenograft in nude mouse. We further showed that ZNF282 is required for E2F1-mediated gene expression in ESCC cells. Thus, ZNF282 is E2F1 co-activator involved in ESCC and elevated expression of ZNF282 is an independent adverse prognostic factor in ESCC.
|
| 巻・号 |
5(23)
|
| ページ |
12260-72
|
| 公開日 |
2014-12-15
|
| DOI |
10.18632/oncotarget.2630
|
| PII |
2630
|
| PMID |
25373738
|
| PMC |
PMC4323012
|
| MeSH |
Aged
Animals
Apoptosis / physiology
Blotting, Western
Carcinoma, Squamous Cell / mortality
Carcinoma, Squamous Cell / pathology*
Cell Movement / physiology
Chromatin Immunoprecipitation
Coenzymes
DNA-Binding Proteins / metabolism*
E2F1 Transcription Factor / metabolism
Esophageal Neoplasms / mortality
Esophageal Neoplasms / pathology*
Esophageal Squamous Cell Carcinoma
Female
Gene Knockdown Techniques
Heterografts
Humans
Immunohistochemistry
Kaplan-Meier Estimate
Male
Mice
Mice, Inbred NOD
Mice, SCID
Middle Aged
Prognosis
Real-Time Polymerase Chain Reaction
Tissue Array Analysis
Transfection
Zinc Fingers / physiology*
|
| IF |
5.168
|
| リソース情報 |
| ヒト・動物細胞 |
TE-1(RCB1894)
TE-4(RCB2097)
TE-5(RCB1949)
TE-8(RCB2098)
TE-9(RCB1988)
TE-10(RCB2099)
TE-11(RCB2100)
TE-14(RCB2101)
TE-15(RCB1951) |
