| RRC ID |
72940
|
| 著者 |
Birot A, Kus K, Priest E, Al Alwash A, Castello A, Mohammed S, Vasiljeva L, Kilchert C.
|
| タイトル |
RNA-binding protein Mub1 and the nuclear RNA exosome act to fine-tune environmental stress response.
|
| ジャーナル |
Life Sci Alliance
|
| Abstract |
The nuclear RNA exosome plays a key role in controlling the levels of multiple protein-coding and non-coding RNAs. Recruitment of the exosome to specific RNA substrates is mediated by RNA-binding co-factors. The transient interaction between co-factors and the exosome as well as the rapid decay of RNA substrates make identification of exosome co-factors challenging. Here, we use comparative poly(A)+ RNA interactome capture in fission yeast expressing three different mutants of the exosome to identify proteins that interact with poly(A)+ RNA in an exosome-dependent manner. Our analyses identify multiple RNA-binding proteins whose association with RNA is altered in exosome mutants, including the zinc-finger protein Mub1. Mub1 is required to maintain the levels of a subset of exosome RNA substrates including mRNAs encoding for stress-responsive proteins. Removal of the zinc-finger domain leads to loss of RNA suppression under non-stressed conditions, altered expression of heat shock genes in response to stress, and reduced growth at elevated temperature. These findings highlight the importance of exosome-dependent mRNA degradation in buffering gene expression networks to mediate cellular adaptation to stress.
|
| 巻・号 |
5(2)
|
| 公開日 |
2022-2-1
|
| DOI |
10.26508/lsa.202101111
|
| PII |
5/2/e202101111
|
| PMID |
34848435
|
| PMC |
PMC8645331
|
| MeSH |
Exosome Multienzyme Ribonuclease Complex / metabolism*
Gene Expression Regulation, Fungal
Gene-Environment Interaction
RNA, Messenger / genetics*
RNA, Messenger / metabolism
RNA, Nuclear / genetics*
RNA, Nuclear / metabolism
RNA-Binding Proteins / metabolism*
Schizosaccharomyces / genetics*
Schizosaccharomyces / metabolism*
Stress, Physiological*
|
| IF |
2.622
|
| リソース情報 |
| 酵母 |
FY9118 |
