RRC ID |
73106
|
著者 |
Brukman NG, Nakajima KP, Valansi C, Flyak K, Li X, Higashiyama T, Podbilewicz B.
|
タイトル |
A novel function for the sperm adhesion protein IZUMO1 in cell-cell fusion.
|
ジャーナル |
J Cell Biol
|
Abstract |
Mammalian sperm-egg adhesion depends on the trans-interaction between the sperm-specific type I glycoprotein IZUMO1 and its oocyte-specific GPI-anchored receptor JUNO. However, the mechanisms and proteins (fusogens) that mediate the following step of gamete fusion remain unknown. Using live imaging and content mixing assays in a heterologous system and structure-guided mutagenesis, we unveil an unexpected function for IZUMO1 in cell-to-cell fusion. We show that IZUMO1 alone is sufficient to induce fusion, and that this ability is retained in a mutant unable to bind JUNO. On the other hand, a triple mutation in exposed aromatic residues prevents this fusogenic activity without impairing JUNO interaction. Our findings suggest a second function for IZUMO1 as a unilateral mouse gamete fusogen.
|
巻・号 |
222(2)
|
公開日 |
2023-2-6
|
DOI |
10.1083/jcb.202207147
|
PII |
213693
|
PMID |
36394541
|
PMC |
PMC9671554
|
MeSH |
Animals
Cell Fusion
Immunoglobulins* / metabolism
Male
Membrane Proteins* / metabolism
Mice
Receptors, Cell Surface* / metabolism
Semen / metabolism
Sperm-Ovum Interactions*
Spermatozoa / metabolism
|
IF |
8.811
|
リソース情報 |
ヒト・動物細胞 |
BHK-21(RCB1423) |