| RRC ID |
73763
|
| 著者 |
Lu K, Wazawa T, Sakamoto J, Vu CQ, Nakano M, Kamei Y, Nagai T.
|
| タイトル |
Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature Imaging with a Genetically Encoded Nanothermometer.
|
| ジャーナル |
Nano Lett
|
| Abstract |
Despite improved sensitivity of nanothermometers, direct observation of heat transport inside single cells has remained challenging for the lack of high-speed temperature imaging techniques. Here, we identified insufficient temperature resolution under short signal integration time and slow sensor kinetics as two major bottlenecks. To overcome the limitations, we developed B-gTEMP, a nanothermometer based on the tandem fusion of mNeonGreen and tdTomato fluorescent proteins. We visualized the propagation of heat inside intracellular space by tracking the temporal variation of local temperature at a time resolution of 155 μs and a temperature resolution 0.042 °C. By comparing the fast in situ temperature dynamics with computer-simulated heat diffusion, we estimated the thermal diffusivity of live HeLa cells. The present thermal diffusivity in cells was about 1/5.3 of that of water and much smaller than the values reported for bulk tissues, which may account for observations of heterogeneous intracellular temperature distributions.
|
| 巻・号 |
22(14)
|
| ページ |
5698-5707
|
| 公開日 |
2022-7-27
|
| DOI |
10.1021/acs.nanolett.2c00608
|
| PMID |
35792763
|
| PMC |
PMC9335883
|
| MeSH |
HeLa Cells
Hot Temperature*
Humans
Temperature
Water*
|
| IF |
11.238
|
| リソース情報 |
| 遺伝子材料 |
B-gTEMP/pRSET-b (RDB19859)
B-gTEMP/pcDNA3 (RDB19860) |
