論文 - 詳細
| RRC ID | 73842 |
|---|---|
| 著者 | Tanikawa T, Kitamura M, Hayashi Y, Tomida N, Uwaya A, Isami F, Yokogawa T, Inoue Y. |
| タイトル | Anti-inflammatory Effect of a Combination of Cannabidiol and Morinda citrifolia Extract on Lipopolysaccharide-stimulated RAW264 Macrophages. |
| ジャーナル | In Vivo |
| Abstract |
BACKGROUND/AIM:The inflammatory response plays an important role in the activation and progression of many inflammation-related diseases. Cannabis sativa and Morinda citrifolia have long been used in folk medicine to treat inflammation. Cannabidiol is the most abundant non-psychoactive phytocannabinoid in C. sativa and exhibits anti-inflammatory activity. The objective of this study was to examine the anti-inflammatory effect of cannabidiol in combination with M. citrifolia and compare its effects with those of cannabidiol alone. MATERIALS AND METHODS:RAW264 cells stimulated with lipopolysaccharide (200 ng/ml) were treated with cannabidiol (0-10 μM), M. citrifolia seed extract (0-100 μg/ml), or a combination of both for 8 or 24 h. Following the treatments, nitric oxide production in the activated RAW264 cells and the expression of inducible nitric oxide synthase were assessed. RESULTS:Our results showed that combination of cannabidiol (2.5 μM) and M. citrifolia seed extract (100 μg/ml) exhibited more efficient inhibition of nitric oxide production than cannabidiol treatment alone in lipopolysaccharide-stimulated RAW264 cells. The combination treatment also reduced the expression of inducible nitric oxide synthase. CONCLUSION:These results suggest that the anti-inflammatory effect of combined treatment with cannabidiol and M. citrifolia seed extract causes a reduction in the expression of inflammatory mediators. |
| 巻・号 | 37(2) |
| ページ | 591-595 |
| 公開日 | 2023-1-1 |
| DOI | 10.21873/invivo.13117 |
| PII | 37/2/591 |
| PMID | 36881078 |
| MeSH | Cannabidiol* / pharmacology Inflammation / drug therapy Lipopolysaccharides Macrophages Morinda* Nitric Oxide Nitric Oxide Synthase Type II Plant Extracts / pharmacology |
| IF | 1.541 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |