| RRC ID |
74467
|
| 著者 |
Yasudome R, Seki N, Asai S, Goto Y, Kita Y, Hozaka Y, Wada M, Tanabe K, Idichi T, Mori S, Ohtsuka T.
|
| タイトル |
Molecular Pathogenesis of Colorectal Cancer: Impact of Oncogenic Targets Regulated by Tumor Suppressive miR-139-3p.
|
| ジャーナル |
Int J Mol Sci
|
| Abstract |
We recently determined the RNA sequencing-based microRNA (miRNA) expression signature of colorectal cancer (CRC). Analysis of the signature showed that the expression of both strands of pre-miR-139 (miR-139-5p, the guide strand, and miR-139-3p, the passenger strand) was significantly reduced in CRC tissues. Transient transfection assays revealed that expression of miR-139-3p blocked cancer cell malignant transformation (e.g., cell proliferation, migration, and invasion). Notably, expression of miR-139-3p markedly blocked RAC-alpha serine/threonine-protein kinase (AKT) phosphorylation in CRC cells. A combination of in silico database and gene expression analyses of miR-139-3p-transfected cells revealed 29 putative targets regulated by miR-139-3p in CRC cells. RNA immunoprecipitation analysis using an Argonaute2 (AGO2) antibody revealed that KRT80 was efficiently incorporated into the RNA-induced silencing complex. Aberrant expression of Keratin 80 (KRT80) was detected in CRC clinical specimens by immunostaining. A knockdown assay using small interfering RNA (siRNA) targeting KRT80 showed that reducing KRT80 expression suppressed the malignant transformation (cancer cell migration and invasion) of CRC cells. Importantly, inhibiting KRT80 expression reduced AKT phosphorylation in CRC cells. Moreover, hexokinase-2 (HK2) expression was reduced in cells transfected with the KRT80 siRNAs or miR-139-3p. The involvement of miRNA passenger strands (e.g., miR-139-3p) in CRC cells is a new concept in miRNA studies. Our tumor-suppressive miRNA-based approach helps elucidate the molecular pathogenesis of CRC.
|
| 巻・号 |
23(19)
|
| 公開日 |
2022-10-1
|
| DOI |
10.3390/ijms231911616
|
| PII |
ijms231911616
|
| PMID |
36232922
|
| PMC |
PMC9569794
|
| MeSH |
Cell Line, Tumor
Cell Movement / genetics
Cell Proliferation / genetics
Cell Transformation, Neoplastic / genetics
Colorectal Neoplasms* / genetics
Colorectal Neoplasms* / pathology
Gene Expression Regulation, Neoplastic
Hexokinase / metabolism
Humans
Keratins / metabolism
MicroRNAs* / genetics
MicroRNAs* / metabolism
Proto-Oncogene Proteins c-akt / metabolism
RNA, Small Interfering
RNA-Induced Silencing Complex / genetics
Serine / metabolism
Threonine / metabolism
|
| IF |
4.556
|
| リソース情報 |
| ヒト・動物細胞 |
HCT116(RCB2979) |
