| RRC ID |
77011
|
| 著者 |
Sugiyama M, Baek SY, Takashima S, Miyashita N, Ishida K, Mun J, Yeo SH.
|
| タイトル |
Overexpression of PkINO1 improves ethanol resistance of Pichia kudriavzevii N77-4 isolated from the Korean traditional fermentation starter nuruk.
|
| ジャーナル |
J Biosci Bioeng
|
| Abstract |
The yeast Pichia kudriavzevii N77-4 was isolated from the Korean traditional fermentation starter nuruk. In this study, fermentation performance and stress resistance ability of N77-4 was analyzed. N77-4 displayed superior thermotolerance (up to 44°C) in addition to enhanced acetic acid resistance compared to Saccharomyces cerevisiae. Moreover, N77-4 produced 7.4 g/L of ethanol with an overall production yield of 0.37 g/g glucose in 20 g/L glucose medium. However, in 250 g/L glucose medium the growth of N77-4 slowed down when the concentration of ethanol reached 14 g/L or more and ethanol production yield also decreased to 0.30 g/g glucose. An ethanol sensitivity test indicated that N77-4 was sensitive to the presence of 1% ethanol, which was not the case for S. cerevisiae. Furthermore, N77-4 displayed a severe growth defect in the presence of 6% ethanol. Because inositol biosynthesis is critical for ethanol resistance, expression levels of the PkINO1 encoding a key enzyme for inositol biosynthesis was analyzed under ethanol stress conditions. We found that ethanol stress clearly repressed PkINO1 expression in a dose-dependent manner and overexpression of PkINO1 improved the growth of N77-4 by 19% in the presence of 6% ethanol. Furthermore, inositol supplementation also enhanced the growth by 13% under 6% ethanol condition. These findings indicate that preventing downregulation in PkINO1 expression caused by ethanol stress improves ethanol resistance and enhances the utility of P. kudriavzevii N77-4 in brewing and fermentation biotechnology.
|
| 巻・号 |
126(6)
|
| ページ |
682-689
|
| 公開日 |
2018-12-1
|
| DOI |
10.1016/j.jbiosc.2018.06.001
|
| PII |
S1389-1723(17)31268-9
|
| PMID |
30401451
|
| MeSH |
Acetic Acid / metabolism
Bioreactors*
Drug Resistance, Fungal / genetics*
Ethanol / metabolism
Ethanol / toxicity*
Fermentation / genetics*
Glucose / metabolism
Metabolic Engineering / methods
Organisms, Genetically Modified
Phosphoric Monoester Hydrolases / genetics*
Phosphoric Monoester Hydrolases / metabolism
Pichia* / genetics
Pichia* / metabolism
Republic of Korea
Thermotolerance / genetics
Up-Regulation / genetics
|
| IF |
2.366
|
| リソース情報 |
| 酵母 |
BY29039 |
