Reference - Detail
| RRC ID | 78442 |
|---|---|
| Author | Fujibayashi S, Kiyono T, Endo Y, Tani T, Tate H, Bai L, Sugano E, Tomita H, Fukuda T. |
| Title | Increased lentivirus titer using ultra expression vectors. |
| Journal | Anal Biochem |
| Abstract |
Lentivirus is an efficient gene transfer system that is widely used in basic science. We aimed to improve viral titer by applying an ultra-expression vectors to lentiviral packaging. Application of the ultra-expression vectors increased biological titer 4 times for standard preparation. We also evaluated the efficacy of the ultra-expression vectors to relatively longer insert fragments, such as CSII-CMV-CNROE containing 5 genes in multiple cloning sites. Packaging of the ultra-expression vectors showed 3.5 times higher biological titer compared with the original method. Our improved packaging system could be applied to lentivirus to produce higher titers. |
| Volume | 669 |
| Pages | 115119 |
| Published | 2023-5-15 |
| DOI | 10.1016/j.ab.2023.115119 |
| PII | S0003-2697(23)00084-2 |
| PMID | 36958509 |
| MeSH | Base Sequence Genetic Vectors* / genetics Lentivirus* / genetics Lentivirus* / metabolism Transduction, Genetic |
| Resource | |
| Human and Animal Cells | HE16(RCB2237) |