論文 - 詳細
| RRC ID | 78444 |
|---|---|
| 著者 | Bartho LA, Kandel M, Walker SP, Cluver CA, Hastie R, Bergman L, Pritchard N, Cannon P, Nguyen TV, Wong GP, MacDonald TM, Keenan E, Hannan NJ, Tong S, Kaitu'u-Lino TJ. |
| タイトル | Circulating Chemerin Is Elevated in Women With Preeclampsia. |
| ジャーナル | Endocrinology |
| Abstract |
BACKGROUND:Preeclampsia is a severe complication of pregnancy. Chemerin is an adipokine secreted from adipose tissue and highly expressed in placenta. This study evaluated the biomarker potential of circulating chemerin to predict preeclampsia. METHODS:Maternal plasma and placenta were collected from women with early-onset preeclampsia (<34 weeks), with preeclampsia and eclampsia, or before preeclampsia diagnosis (36 weeks). Human trophoblast stem cells were differentiated into syncytiotrophoblast or extravillous trophoblasts across 96 hours. Cells were cultured in 1% O2 (hypoxia) or 5% O2 (normoxia). Chemerin was measured by enzyme-linked immunosorbent assay (ELISA) and RARRES2 (gene coding chemerin) by reverse transcription-quantitative polymerase chain reaction. RESULTS:Circulating chemerin was increased in 46 women with early-onset preeclampsia (<34 weeks) compared to 17 controls (P < .0006). Chemerin was increased in placenta from 43 women with early-onset preeclampsia compared to 24 controls (P < .0001). RARRES2 was reduced in placenta from 43 women with early-onset preeclampsia vs 24 controls (P < .0001). Chemerin was increased in plasma from 26 women with established preeclampsia (P = .006), vs 15 controls. Circulating chemerin was increased in 23 women who later developed preeclampsia vs 182 who did not (P = 3.23 × 10-6). RARRES2 was reduced in syncytiotrophoblast (P = .005) or extravillous trophoblasts (P < .0001). Hypoxia increased RARRES2 expression in syncytiotrophoblast (P = .01) but not cytotrophoblast cells. CONCLUSIONS:Circulating chemerin was elevated in women with early-onset preeclampsia, established preeclampsia, and preceding preeclampsia diagnosis of preeclampsia. RARRES2 was dysregulated in placenta complicated by preeclampsia and may be regulated through hypoxia. Chemerin may have potential as a biomarker for preeclampsia but would need to be combined with other biomarkers. |
| 巻・号 | 164(5) |
| 公開日 | 2023-3-13 |
| DOI | 10.1210/endocr/bqad041 |
| PII | 7071694 |
| PMID | 36882076 |
| PMC | PMC10032305 |
| MeSH | Biomarkers / metabolism Female Humans Hypoxia / metabolism Placenta / metabolism Pre-Eclampsia* / diagnosis Pregnancy Trophoblasts / metabolism |
| リソース情報 | |
| ヒト・動物細胞 | CT27(RCB4936) CT29(RCB4937) CT30(RCB4938) bTS5(RCB4940) bTS11(RCB4941) |