| RRC ID |
78556
|
| 著者 |
Taketoshi Futatsuya, Tatsuki Mura, Kazushi Anzawa, Takashi Mochizuki, Akira Shimizu, Yoshitsugu Iinuma
|
| タイトル |
MALDI‐TOF MS identification of Exophiala species isolated in Japan: Library enrichment and faster sample preparation
|
| ジャーナル |
The Journal of Dermatology
|
| Abstract |
Exophiala species cause chromoblastomycosis, mycetoma, and phaeohyphomycosis, which are occasionally fatally in immunocompromised patients. Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) provides rapid and accurate examination of isolated bacteria and some fungal isolates, but the preparation method for filamentous fungi is complicated. In this study, 31 clinical isolates of Exophiala spp. in Japan were identified by MALDI‐TOF MS with a library enriched by adding data. To simplify the sample preparation method, two modified methods were compared with the standard method for filamentous fungi. The agar cultivation sample preparation method reduced the time required for liquid culture and was considered suitable for clinical use. In 30 of 31 clinical isolates of Exophiala spp., the species identified by MALDI‐TOF MS with the highest score matched the species identified by sequencing the internal transcribed spacer region. Exophiala dermatitidis, E. lecanii‐corni, and E. oligosperma were identified above the genus level, while E. jeanselmei and E. xenobiotica were often not identified at the species level. The identification scores tended to be lower for less‐registered strains in the in‐house library. It is suggested that library enrichment and the modified preparation method may facilitate early diagnosis of rare fungal infections by Exophiala spp. in clinical laboratories using MALDI‐TOF MS.
|
| 巻・号 |
50
|
| ページ |
1313-1320
|
| 公開日 |
2023-10-1
|
| DOI |
10.1111/1346-8138.16878
|
| PMID |
37381719
|
| MeSH |
Exophiala*
Fungi
Humans
Japan
Mycoses*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
|
| リソース情報 |
| 病原真核微生物 |
Exophiala bergeri IFM 4870, Exophiala cancerae IFM 4973, IFM 4979, IFM 4975, Exophiala dermatitidis IFM 4827, IFM 4962, IFM 4829, IFM 4847, IFM 4850, Exophiala equina IFM 4971, Exophiala jeanselmei IFM 4866, IFM 4852, Exophiala lecanii-corni IFM 4970, IFM 4981, Exophiala monilae IFM 41500, Exophiala nishimurae IFM 41855, Exophiala oligosperma IFM 57491, IFM 4982, IFM 4871, IFM 4858, IFM 4868, IFM 4983, Exophiala salmonis IFM 4884, Exophiala spinifera IFM 41507, IFM 41514, IFM 41515, IFM 41703, Exophiala xenobiotica IFM 41496, IFM 4857, Fonsecaea pedrosoi IFM 4887, IFM 46433, Hortaea werneckii IFM 41542 |
