| RRC ID |
78567
|
| 著者 |
Dibwe DF, Takeishi N, Oba S, Sakurai A, Sakurai T, Tsukui T, Chiba H, Hui SP.
|
| タイトル |
Identification of a β-Carboline Alkaloid from Chemoselectively Derived Vanilla Bean Extract and Its Prevention of Lipid Droplet Accumulation in Human Hepatocytes (HepG2).
|
| ジャーナル |
Molecules
|
| Abstract |
Targeting bioactive compounds to prevent lipid droplet accumulation in the liver, we explored an antioxidative extract from vanilla bean (Vainilla planifolia) after chemo-selective derivatization through heating and acid modification. The chemical analysis of vanilla bean extract through chemoselective derivatization resulted in the identification of sixteen compounds (34-50) using LC-MS/MS analysis. A β-carboline alkaloid with a piperidine C-ring and a vanillin moiety at C-1 (34) was identified by molecular networking and diagnostic fragmentation filtering approaches. β-carboline alkaloid 34 exhibited significant inhibitory activity of lipid droplet accumulation (LDAI) in oleic acid-loaded hepatocellular carcinoma HepG2 cells. The LDAI activity was associated with both activation of lipolysis and suppression of lipogenesis in the cells. The study indicates that crude plant extracts, following chemoselective derivatization, may contain bioactive compounds that could be beneficial in preventing hepatosteatosis and could serve as a source of lead compounds for drug development. This approach may be useful to investigate other mixtures of natural products and food resources.
|
| 巻・号 |
28(24)
|
| 公開日 |
2023-12-9
|
| DOI |
10.3390/molecules28248024
|
| PII |
molecules28248024
|
| PMID |
38138514
|
| PMC |
PMC10745329
|
| MeSH |
Alkaloids* / pharmacology
Carbolines / pharmacology
Chromatography, Liquid
Hep G2 Cells
Humans
Lipid Droplets
Plant Extracts / chemistry
Plant Extracts / pharmacology
Tandem Mass Spectrometry
Vanilla* / chemistry
|
| リソース情報 |
| ヒト・動物細胞 |
Hep G2 |
