| RRC ID |
78879
|
| 著者 |
Tsuchiya M, Tachibana N, Hamachi I.
|
| タイトル |
Post-click labeling enables highly accurate single cell analyses of glucose uptake ex vivo and in vivo.
|
| ジャーナル |
Commun Biol
|
| Abstract |
Cellular glucose uptake is a key feature reflecting metabolic demand of cells in physiopathological conditions. Fluorophore-conjugated sugar derivatives are widely used for monitoring glucose transporter (GLUT) activity at the single-cell level, but have limitations in in vivo applications. Here, we develop a click chemistry-based post-labeling method for flow cytometric measurement of glucose uptake with low background adsorption. This strategy relies on GLUT-mediated uptake of azide-tagged sugars, and subsequent intracellular labeling with a cell-permeable fluorescent reagent via a copper-free click reaction. Screening a library of azide-substituted monosaccharides, we discover 6-azido-6-deoxy-D-galactose (6AzGal) as a suitable substrate of GLUTs. 6AzGal displays glucose-like physicochemical properties and reproduces in vivo dynamics similar to 18F-FDG. Combining this method with multi-parametric immunophenotyping, we demonstrate the ability to precisely resolve metabolically-activated cells with various GLUT activities in ex vivo and in vivo models. Overall, this method provides opportunities to dissect the heterogenous metabolic landscape in complex tissue environments.
|
| 巻・号 |
7(1)
|
| ページ |
459
|
| 公開日 |
2024-4-16
|
| DOI |
10.1038/s42003-024-06164-y
|
| PII |
10.1038/s42003-024-06164-y
|
| PMID |
38627603
|
| PMC |
PMC11021395
|
| MeSH |
Azides* / chemistry
Glucose* / metabolism
Single-Cell Analysis
|
| リソース情報 |
| ヒト・動物細胞 |
K562(RCB0027) |
