| RRC ID |
78880
|
| 著者 |
Saito K, Toyoda H, Okada M, Oh JS, Nakazawa K, Ban Y, Orita K, Shimatani A, Yao H, Shirafuji T, Nakamura H.
|
| タイトル |
Fracture healing on non-union fracture model promoted by non-thermal atmospheric-pressure plasma.
|
| ジャーナル |
PLoS One
|
| Abstract |
Non-thermal atmospheric-pressure plasma (NTAPP) is attracting widespread interest for use in medical applications. The tissue repair capacity of NTAPP has been reported in various fields; however, little is known about its effect on fracture healing. Non-union or delayed union after a fracture is a clinical challenge. In this study, we aimed to investigate how NTAPP irradiation promotes fracture healing in a non-union fracture model and its underlying mechanism, in vitro and in vivo. For the in vivo study, we created normal and non-union fracture models in LEW/SsNSlc rats to investigate the effects of NTAPP. To create a fracture, a transverse osteotomy was performed in the middle of the femoral shaft. To induce the non-union fracture model, the periosteum surrounding the fracture site was cauterized after a normal fracture model was created. The normal fracture model showed no significant difference in bone healing between the control and NTAPP-treated groups. The non-union fracture model demonstrated that the NTAPP-treated group showed consistent improvement in fracture healing. Histological and biomechanical assessments confirmed the fracture healing. The in vitro study using pre-osteoblastic MC3T3-E1 cells demonstrated that NTAPP irradiation under specific conditions did not reduce cell proliferation but did enhance osteoblastic differentiation. Overall, these results suggest that NTAPP is a novel approach to the treatment of bone fractures.
|
| 巻・号 |
19(4)
|
| ページ |
e0298086
|
| 公開日 |
2024-1-1
|
| DOI |
10.1371/journal.pone.0298086
|
| PII |
PONE-D-23-33924
|
| PMID |
38626076
|
| PMC |
PMC11020618
|
| MeSH |
Animals
Cell Differentiation
Cell Proliferation
Femoral Fractures* / surgery
Fracture Healing
Fractures, Bone*
Plasma Gases* / pharmacology
Plasma Gases* / therapeutic use
Rats
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
