| RRC ID |
78955
|
| 著者 |
Kubo T, Yamagata Y, Matsusaka H, Toyoda A, Sato Y, Kumamaru T.
|
| タイトル |
MiRiQ Database: A Platform for In Silico Rice Mutant Screening.
|
| ジャーナル |
Plant Cell Physiol
|
| Abstract |
Genetic studies using mutant resources have significantly contributed to elucidating plant gene function. Massive mutant libraries sequenced by next-generation sequencing technology facilitate mutant identification and functional analysis of genes of interest. Here, we report the creation and release of an open-access database (https://miriq.agr.kyushu-u.ac.jp/index.php), called Mutation-induced Rice in Kyushu University (MiRiQ), designed for in silico mutant screening based on a whole-genome-sequenced mutant library. This database allows any user to easily find mutants of interest without laborious efforts such as large-scale screening by PCR. The initial version of the MiRiQ database (version 1.0) harbors a total of 1.6 million single-nucleotide variants (SNVs) and InDels of 721 M1 plants that were mutagenized by N-methyl-N-nitrosourea treatment of the rice cultivar Nipponbare (Oryza sativa ssp. japonica). The SNVs were distributed among 87% of all 35,630 annotated protein-coding genes of the Nipponbare genome and were predicted to induce missense and nonsense mutations. The MiRiQ database provides built-in tools, such as a search tool by keywords and JBrowse for mutation searches. Users can request mutant seeds in the M2 or M3 generations from a request form linked to this database. We believe that the availability of a wide range of gene mutations in this database will benefit the plant science community and breeders worldwide by accelerating functional genomic research and crop improvement.
|
| 巻・号 |
65(1)
|
| ページ |
169-174
|
| 公開日 |
2024-1-19
|
| DOI |
10.1093/pcp/pcad134
|
| PII |
7335587
|
| PMID |
37930817
|
| PMC |
PMC10799713
|
| MeSH |
Base Sequence
Genes, Plant
Genome, Plant / genetics
Humans
Mutation / genetics
Oryza* / genetics
|
| リソース情報 |
| イネ |
Induced mutation lines |
