論文 - 詳細
| RRC ID | 79126 |
|---|---|
| 著者 | Inoue Y, Suzuki Y, Kunishima Y, Washio T, Morishita S, Takeda H. |
| タイトル | High-fat diet in early life triggers both reversible and persistent epigenetic changes in the medaka fish (Oryzias latipes). |
| ジャーナル | BMC Genomics |
| Abstract |
BACKGROUND:The nutritional status during early life can have enduring effects on an animal's metabolism, although the mechanisms underlying these long-term effects are still unclear. Epigenetic modifications are considered a prime candidate mechanism for encoding early-life nutritional memories during this critical developmental period. However, the extent to which these epigenetic changes occur and persist over time remains uncertain, in part due to challenges associated with directly stimulating the fetus with specific nutrients in viviparous mammalian systems. RESULTS:In this study, we used medaka as an oviparous vertebrate model to establish an early-life high-fat diet (HFD) model. Larvae were fed with HFD from the hatching stages (one week after fertilization) for six weeks, followed by normal chow (NC) for eight weeks until the adult stage. We examined the changes in the transcriptomic and epigenetic state of the liver over this period. We found that HFD induces simple liver steatosis, accompanied by drastic changes in the hepatic transcriptome, chromatin accessibility, and histone modifications, especially in metabolic genes. These changes were largely reversed after the long-term NC, demonstrating the high plasticity of the epigenetic state in hepatocytes. However, we found a certain number of genomic loci showing non-reversible epigenetic changes, especially around genes related to cell signaling, liver fibrosis, and hepatocellular carcinoma, implying persistent changes in the cellular state of the liver triggered by early-life HFD feeding. CONCLUSION:In summary, our data show that early-life HFD feeding triggers both reversible and persistent epigenetic changes in medaka hepatocytes. Our data provide novel insights into the epigenetic mechanism of nutritional programming and a comprehensive atlas of the long-term epigenetic state in an early-life HFD model of non-mammalian vertebrates. |
| 巻・号 | 24(1) |
| ページ | 472 |
| 公開日 | 2023-8-21 |
| DOI | 10.1186/s12864-023-09557-1 |
| PII | 10.1186/s12864-023-09557-1 |
| PMID | 37605229 |
| PMC | PMC10441761 |
| MeSH | Animals Diet, High-Fat Epigenesis, Genetic* Gene Expression Regulation, Developmental Genetic Loci Oryzias / genetics Oryzias / growth & development |
| リソース情報 | |
| メダカ | Hi-medaka |