| RRC ID |
79163
|
| 著者 |
Wattrus SJ, Smith ML, Rodrigues CP, Hagedorn EJ, Kim JW, Budnik B, Zon LI.
|
| タイトル |
Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality.
|
| ジャーナル |
Science
|
| Abstract |
Tissue-specific stem cells persist for a lifetime and can differentiate to maintain homeostasis or transform to initiate cancer. Despite their importance, there are no described quality assurance mechanisms for newly formed stem cells. We observed intimate and specific interactions between macrophages and nascent blood stem cells in zebrafish embryos. Macrophage interactions frequently led to either removal of cytoplasmic material and stem cell division or complete engulfment and stem cell death. Stressed stem cells were marked by surface Calreticulin, which stimulated macrophage interactions. Using cellular barcoding, we found that Calreticulin knock-down or embryonic macrophage depletion reduced the number of stem cell clones that established adult hematopoiesis. Our work supports a model in which embryonic macrophages determine hematopoietic clonality by monitoring stem cell quality.
|
| 巻・号 |
377(6613)
|
| ページ |
1413-1419
|
| 公開日 |
2022-9-23
|
| DOI |
10.1126/science.abo4837
|
| PMID |
36137040
|
| PMC |
PMC9524573
|
| MeSH |
Animals
Apoptosis*
Calbindin 2 / genetics
Calbindin 2 / physiology
Calreticulin* / genetics
Calreticulin* / metabolism
Cell Communication*
Clonal Hematopoiesis* / genetics
Clonal Hematopoiesis* / physiology
Embryo, Nonmammalian
Hematopoietic Stem Cells* / physiology
Macrophages* / physiology
Zebrafish
Zebrafish Proteins / genetics
Zebrafish Proteins / physiology
|
| リソース情報 |
| ゼブラフィッシュ |
Tg(EF1α:mAG-zGem(1/100))rw0410h |
