RRC ID |
997
|
著者 |
Sharmin S, Ohori A, Sano A, Kamei K, Yamaguchi M, Takeo K, Uno J, Nishimura K, Miyaji M.
|
タイトル |
Histoplasma capsulatum variety duboisii isolated in Japan from an HIV-infected Ugandan patient.
|
ジャーナル |
Nihon Ishinkin Gakkai Zasshi
|
Abstract |
A strain of Histoplasma capsulatum var. duboisii (deposited as IFM 50954 in Chiba University) was isolated from the cerebrospinal fluid of a female Ugandan patient infected with HIV. The isolate had in vitro urease activity on Christensen's urea agar slants, although the common belief is that H. capsulatum var. duboisii is urease negative, and is, considered one of the characteristic markers that distinguishes the three varieties of H. capsulatum. Forty H. capsulatum var. capsulatum, five H. capsulatum var. duboisii, and five H. capsulatum var. farciminosum isolates were evaluated for urease activity on Christensen's urea agar slants and for other qualitative and quantitative urease assays of activity. All 50 isolates of H. capsulatum used in this study were positive for urease activity, suggesting that urease activity may be universal characteristic of H. capsulatum. We also compared the urease activity and pathogenicity of seven H. capsulatum isolates that convert into yeast-form cells. Although isolate IFM 50954 showed moderate virulence in mice and moderate urease activity among tested H. capsulatum isolates, there was no correlation between level of urease activity and pathogenicity. In addition, scanning electron microscopy revealed that some microconidia of isolate IFM 50954 formed "double-cell" configurations that were attached to each other by narrow bases.
|
巻・号 |
44(4)
|
ページ |
299-306
|
公開日 |
2003-1-1
|
DOI |
10.3314/jjmm.44.299
|
PMID |
14615797
|
MeSH |
Animals
Female
HIV Infections / microbiology*
Histoplasma / isolation & purification*
Histoplasma / pathogenicity
Humans
Japan
Male
Mice
Mice, Inbred Strains
Uganda / ethnology
Urease / analysis
|
リソース情報 |
病原微生物 |
IFM 5396
IFM 5397
IFM 5398
IFM 5399
IFM 5400
IFM 5401
IFM 5402
IFM 5403
IFM 5404
IFM 5405
IFM 5406
IFM 5407
IFM 5408
IFM 5410
IFM 5411
IFM 5412
IFM 5413
IFM 5414
IFM 5415
IFM 5416
IFM 5417
IFM 5418
IFM 5844
IFM 40009
IFM 40752
IFM 41323
IFM 41329
IFM 41330
IFM 41331
IFM 41332
IFM 41333
IFM 41334
IFM 41335
IFM 41612
IFM 41618
IFM 41619
IFM 46003
IFM 46004
IFM 46159
IFM 47750
IFM 49109
IFM 49110
IFM 49200
IFM 49721
IFM 50248
IFM 50249
IFM 50250
IFM 50251
IFM 50954
IFM 50955
IFM 50958
IFM 50959 |