RRC ID 11606
著者 Tanaka R, Takase Y, Kanachi M, Enomoto-Katayama R, Shirai T, Nakagoshi H.
タイトル Notch-, Wingless-, and Dpp-mediated signaling pathways are required for functional specification of Drosophila midgut cells.
ジャーナル Dev Biol
Abstract The mechanisms for cell fate determination have been extensively studied whereas little is known about the mechanism through which functional specificity is established. In the Drosophila midgut, copper cells provide an excellent model system to examine this mechanism. Copper is an essential element for the activity of a number of physiologically important enzymes including Cu/Zn-superoxide dismutase, cytochrome c oxidase, and dopamine-beta-hydroxylase. Drosophila copper cells are involved in two distinct functions, i.e., copper absorption and acid secretion, which are visualized as a fluorescent signal and a color change of a pH indicator dye, respectively. Here we show that the absorptive function is established through two independent pathways, the Notch signaling pathway in adjacent interstitial cells and the Wingless signaling pathway in copper cells. Furthermore, the other function, acid secretion, is regulated through the Decapentaplegic and Wingless signaling pathways in interstitial cells. Our results clearly indicate that normal morphological development is insufficient for functional maturation, and that subsequent functional specification is achieved through several independent pathways. These results provide valuable insights into the molecular mechanism underlying functional specification.
巻・号 304(1)
ページ 53-61
公開日 2007-4-1
DOI 10.1016/j.ydbio.2006.12.018
PII S0012-1606(06)01436-9
PMID 17239848
MeSH Animals Copper / metabolism Digestive System / cytology Digestive System / embryology* Drosophila / embryology* Drosophila Proteins / metabolism* Immunohistochemistry Microscopy, Fluorescence Morphogenesis / physiology* Proto-Oncogene Proteins / metabolism* Receptors, Notch / metabolism* Signal Transduction / physiology* Temperature Wnt1 Protein
IF 2.896
引用数 9
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ DGRC#113060 DGRC#113113 DGRC#113157 DGRC#113370 DGRC#113192 DGRC#104395