RRC ID 15362
著者 Wolfstetter G, Holz A.
タイトル The role of LamininB2 (LanB2) during mesoderm differentiation in Drosophila.
ジャーナル Cell Mol Life Sci
Abstract In Drosophila, four genes encode for laminin subunits and the formation of two laminin heterotrimers has been postulated. We report the identification of mutations in the Drosophila LamininB2 (LanB2) gene that encodes for the only laminin γ subunit and is found in both heterotrimers. We describe their effects on embryogenesis, in particular the differentiation of visceral tissues with respect to the ECM. Analysis of mesoderm endoderm interaction indicates disrupted basement membranes and defective endoderm migration, which finally interferes with visceral myotube stretching. Extracellular deposition of laminin is blocked due to the loss of the LanB2 subunit, resulting in an abnormal distribution of ECM components. Our data, concerning the different function of both trimers during organogenesis, suggest that these trimers might act in a cumulative way and probably at multiple steps during ECM assembly. We also observed genetic interactions with kon-tiki and thrombospondin, indicating a role for laminin during muscle attachment.
巻・号 69(2)
ページ 267-82
公開日 2012-1-1
DOI 10.1007/s00018-011-0652-3
PMID 21387145
MeSH Animals Basement Membrane / embryology Cell Differentiation Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / embryology* Drosophila melanogaster / genetics Extracellular Matrix / genetics Extracellular Matrix / metabolism Laminin / genetics Laminin / metabolism* Mesoderm / embryology* Mesoderm / metabolism Mutation Organogenesis* Thrombospondins / metabolism
IF 6.496
引用数 27
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ショウジョウバエ DGRC#103948